Isolation and Culture of Slime Molds

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Topics: Fungus, Slime mold
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Isolation and Culture of Zoosporic Fungi using Baiting Technique
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Rillera DP , Talibsao K , Talucod AC and Tan P
Department of Biological Sciences, College of Science, University of Santo Tomas, España Street, Manila 108

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Zoosporic fungi belonging to class Oomycetes of Kingdom Chromista and Phylum Chytridiomycota of Kingdom Eumycota, were isolated and cultured in this experiment. Baiting technique was conducted using 6 baits: pollen grains, snake skin, animal dung, shrimp exoskeleton and cockroach,. The morphological and reproductive characterization was also conducted on the isolated zoosporic fungi.
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Introduction
Zoospores are motile, asexual spores having one or more flagella. They may be haploid or diploid and are formed inside a sporangium, discharged from the sporangium to become a free swimming stage, and after the motile stage , the zoospore encysts in a suitable substrate or host. ( Lange & Olson, 1983).
Baiting technique is a method that allows further growth of fungal mycelia on prepared baits. Major groups of fungi isolated using baiting techniques are those from Kingdom Chromista, particularly in the Class Oomycetes. The Oomycetes or “egg fungi”, also called water molds were previously grouped with fungi based on their filamentous growth and the presence of coenocytic hyphae, one of the morphological traits and characteristic of fungi. However, their life cycle, unlike that of many fungi, includes a diploid, asexually or sexually reproducing phase. (Madigan, et al., 2009).
Oomycetes differ from fungi in other fundamental ways as well. The cell walls of oomycetes are typically made of cellulose, not chitin, as for fungi, and they have flagellated cells, which are lacking in all but a few fungi. (Madigan, et al, 2009) Zoospores are



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