Is Tea-Tree Oil Effective in Controlling and Minimising the Growth of the Escherichia Coli Bacteria?

Powerful Essays
QUEENSLAND ACADEMIES FOR SCIENCE, MATHEMATICS AND TECHNOLOGY International Baccalaureate Diploma Program
Extended Essay in Biology

Is Tea-tree Oil effective in controlling and minimising the growth of the Escherichia coli Bacteria?

A study of the effectiveness of a Natural Antiseptic

Candidate Name: Lois Adrienne Dianne Soriano Dulay
Candidate Session Number: 003072-032
Word Count: 3997
Supervisor: Ms Kate Barry
Date of Submission: 25th July 2011
Acknowledgments
Firstly I would like to thank my supervisor, Mrs Kate Barry for her continual support throughout the course of my extended essay. Thank you for our encouraging discussions and for inspiring me to pursue this area of investigation during our Biology classes. Thank you for always making time for me and my Extended Essay whenever I needed your advice and support.
Thank you to Mrs Kerry Bullock, our Extended Essay coordinator, for organising everything to do with the Extended Essay. Your efforts and the motivation you provide for our entire cohort has been exceptional.
Our laboratory technicians, Ms Teneille Gesch and Ms Kathy Pope, for providing me with all of the equipment I needed to carry out my experiment, and for teaching me various and safe techniques with regards to handling live bacteria cultures. Special thanks to Ms Gesch, for giving up several days of her Christmas holidays to supervise me as I conducted my experiment. Your time is greatly appreciated.
Thank you to Mrs Meredith Erbacher, our teacher librarian, for making time to teach me the correct ways of referencing sources. Your knowledge has been very helpful.
My family – thank you for your undying love, support and understanding throughout the completion of this essay. I know that much of my time has been spent on this essay over the past year and that I have missed precious family time which I cannot regain. However, your understanding and encouragement has only motivated me to ensure that the production of this essay



References: "The History of Tea Tree Oil". (1976). Retrieved November 23, 2010, from Thursday Plantation - Australia 's Original: http://www.thursdayplantation.com/page/Tea-Tree-Oil/ All about Cellulitis Infections Alleger, I. (2001, February 1). Herbal Treatments - History and Controversy. Retrieved July 6, 2011, from Townsend Letter for Doctors and Patients: http://www.tldp.com/issue/186/Herbs%20Against%20Cancer.htm Aspres, N., & Freeman, S Baby Centre. (2011, February). Meningitis. Retrieved June 24, 2011, from Baby Centre: http://www.babycentre.co.uk/baby/health/meningitis/ Boyle, A Brown, J. C. (1997, September 16). What the Heck is an E.coli? Retrieved May 5, 2011, from Department of Molecular Biosciences - The University of Kansas: http://people.ku.edu/~jbrown/ecoli.html Crawford, S Dryden, M., Daily, S., & Crouch, M. (2004). A randomized, controlled trial of tea tree topical preparations bersus a standard topical regimen for the clearance of MRSA colonization. Journal of Hospital Infection, Volume 56, Issue 4, 283-286. Esoteric Oils. (2011, January 3). Tea tree Oil Information. Retrieved March 9, 2011, from Esoteric Oils: http://www.essentialoils.co.za/essential-oils/tea-tree.htm Flaxman, D., & Griffiths, P Hill, M. P. (2005, June 2). 2008 ANAT3231 Lecture 02 - Cell Types . Retrieved March 5, 2011, from UNSW Cell Biology: http://cellbiology.med.unsw.edu.au/units/science/lecture0802.htm LaPlante, K Madappa, M. M., & Go, C. H. (2011, March 9). eMedicine from WebMD. Retrieved March 8, 2011, from Escherichia Coli Infections: http://emedicine.medscape.com/article/217485-overview Moss, R Moss, R. W. (2001, February 1). Herbs Against Cancer. Retrieved May 6, 2011, from Herbal Treatments - History and Controversy: http://www.tldp.com/issue/186/Herbs%20Against%20Cancer.htm O 'Brien, P., & Dougherty, T Olsen, C. (1999, January 15). Tea Tree Oil (Melaleuca alternifolia). Retrieved March 8, 2011, from Tea Tree Oil (Melaleuca alternifolia): http://www.se1.us/health/tea-tree/medical.html Pillai, M TypesOfBacteria Website. (2008, November 21). How do Disinfectants and Antiseptics Work? Retrieved March 7, 2011, from Types Of Bacteria: http://www.typesofbacteria.co.uk/how-do-disinfectants-antiseptics-work.html U.S World Health Organisation. (2010, December). Traditional medicine: a global perspective. Retrieved July 6, 2011, from Bulletin of the World Health Organisation: http://www.who.int/bulletin/volumes/88/12/10-079822/en/ (2007) (2007). Microbes and Biotechnology (Chapter 17). In A. Damon, R. McGonegal, P. Tosto, & W. Ward, Higher Level Biology - Developed Specifically for the IB Diploma (pp. 540-541). Essex: Heinmann - Pearson Education Limited.

You May Also Find These Documents Helpful

  • Good Essays

    Tea tree oil has numerous uses in first aid remedies because of its triple antibiotic functions: it is an antiviral, antibacterial, & antifungal agent. The oil's first aid uses are fairly straightforward simply because we all know what to expect from a first aid treatment: we know we can use the substance to sanitize, heal cuts and wounds, & ease pain.…

    • 519 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    The results for the groups that tested tea tree for antibacterial activity, varied greatly. For one group, tea tree’s zone of inhibition measured at 9mm and for another group it measured at 12mm.…

    • 939 Words
    • 4 Pages
    Powerful Essays
  • Good Essays

    Essential oils are extracted from plants, trees and fruits and usually have a particular fragrance. Often they have medicinal or soothing effects that are included in homeopathic remedies - tea tree oil for example is extracted from an Australian plant that was originally used by aboriginals for healing purposes. Eucalyptus is often used in decongestants to clear the sinuses, and could be put to use in an aromatherapy candle for someone suffering from a cold or flu.…

    • 551 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Australian tea tree is the great source of aromatherapy essential oils. This tree has numerous health benefits and effects than any other trees. Every parts of this tree are beneficial to any disease of the human. The leaves of this tree cure the skin diseases of the human and give most attractive skin. In the first aid box, this aromatherapy essential oil has a vital role to cure numerous injuries and diseases. Many people struggle to breathe due to their cold, cough or any sinus problems. These aromatherapy essential oils are very useful in making the people breathing easier and give the peaceful sleep. People can inhale the fragrant essential oil that helps them to open up their air ways from congestion. Aromatherapy essential oils are volatile in nature and it is also known as hydrophobic liquid that is created naturally. Some of the products of essential oils in the market are rose oils, eucalyptus essential oils, and lavender…

    • 450 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Tea tree oil is obtained from the leaves of Melaleuca alternifolia by steam distilling them. The plant mentioned here is a native to Australia.…

    • 443 Words
    • 2 Pages
    Good Essays
  • Good Essays

    The world now is heading more towards natural medications, or medication with natural herbs and fruits. Yes since is on its peak, but yet we want to step away from chemicals and pills, we are indeed looking for something that will do the job without any side effects, we want something that will cure our body without harming it. That thing is totally tea tree oil. From all natural oils, tea tree oil is thought to be the best. For its several benefits and uses, tea tree oil is on the top of the natural oil list. Though some people might confuse the name of tea tree oil with the ordinary tea that we are used to drink, tea tree oil is not the same as green or black tea we drink. Tea tree was mainly discovered by…

    • 1289 Words
    • 6 Pages
    Good Essays
  • Good Essays

    An Essential oil is the fluids from a plant in which it take on the odor and/or taste of the plant one extracts it from. Essential oils have been used through out history dating back to the bible, but had gone cold until 1928 when a French chemist by the name of Rene-Maurice Gattefosse had accidentally burned his hand severely while working. He plunged his hand into the nearest container which happened to be lavender oil instead of Water. To his surprise, his hand did not have any burn scars to be seen. This “ Re-discovery” has opened doors to thousands of uses of essential oils.…

    • 478 Words
    • 2 Pages
    Good Essays
  • Good Essays

    The history of essential oils goes all the way back to the time of the bible. Essential oils that are mentioned in the bible are cedarwood, frankincense, fir, cinnamon, myrrh, myrtle and spikenard. Essential oils can be found in different types of plants. For example, frankincense can be found in a frankincense tree. Today’s recipes/blends for essential oils are a direct result of biblica references. People around the world use essential oils every day to help with illnesses, stress and other uses such as fragrance bathing products, incense, perfumes, and cosmetics.…

    • 274 Words
    • 2 Pages
    Good Essays
  • Good Essays

    aromatherapy

    • 1627 Words
    • 5 Pages

    In the Dordogne of France there are cave paintings found to be 18,000 years old that depict the use of plants for medicinal purposes. The origins of aromatherapy have been lost with time before the keeping of any records. It is although believed that crude forms of distillation, the main method that essential oils are now obtained, were practiced thousands of years ago in Persia, Egypt and India. Distillation was used mainly for the extraction of exotic flower waters.…

    • 1627 Words
    • 5 Pages
    Good Essays
  • Powerful Essays

    Tea Market Analysis in India

    • 2883 Words
    • 12 Pages

    Tea is basically the dried and processed leaves of only one species of plant called camellia sinensis.…

    • 2883 Words
    • 12 Pages
    Powerful Essays
  • Good Essays

    Melaleuca

    • 601 Words
    • 3 Pages

    Melaleuca oil was used long before this company was formed. The Aborigines in Australia discovered a plant called the Melaleuca alternifolia tree and that this plant had healing properties. They used this plant to treat insect bites and heal wounds. It got the nickname tea tree because the leaves from the tree were boiled. Laboratory test were done and soon it was found that this tea tree oil was very effective in treating burns, insect bites, fungal and bacterial infections and other skin conditions. Some used the plant for so many applications that they called it a ‘first- aid kit in a bottle,’ reflected in the fact that Australian soldiers in World War II carried the oil of Melaleuca in their first-aid kits (Net Industries, LLC, 2007).…

    • 601 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    The results of our experiment did not support our hypothesis that cilantro would not have an antimicrobial effect against E. coli due to the bacteria having an outer membrane. Although cilantro did not act as an antibiotic against E. coli, it was probably not because the bacteria have an outer membrane. Research shows that cilantro can in fact affect gram negative bacteria such as Salmonella and E. coli that contain outer membranes. The results of our experiment did not support our hypothesis that cilantro would have an antimicrobial effect on S. epidermis. We hypothesized that because S. epidermis cells do not have an outer membrane, cilantro would be able to penetrate and kill the bacteria. We think there are a few possible explanations for why our results do not support our hypothesis. First, the cilantro might have been contaminated with different kinds of bacteria. This contamination could be the result of the cilantro coming into contact with our hands as we placed it into the mixer. Although we washed our hands, hand washing doesn 't eliminate all bacteria. It only reduces the load of bacteria on the hands (Tortura et al., 2010) the contamination could be the result of using an unsterile mixer. Again, although we washed the mixer before use, it was not sterilized which is the only way to completely eliminate all microbes from an object (Tortura et al., 2010). Finally, the cilantro itself or the bag it was carried in could be the source of contamination. Perhaps someone in the room the day the experiments were conducted coughed or sneezed near the cilantro.…

    • 2091 Words
    • 9 Pages
    Powerful Essays
  • Good Essays

    Escherichia coli is a heterotrophic bacteria that is most commonly found within the small intestines of humans and other animals. This bacteria survives by getting its nutrition through ingesting unused or undigested nutrients in the bowels of its host. If ingested by humans or other mammals, E. coli causes food poisoning and serious infection. There are currently many different strains of E. coli and every living human carries at least one of them in their intestines. Although most strains are harmless there are a few that cause serious ailment. E. coli is a gram negative bacterial cell that consists of a very thin exterior membrane that covers a layer of peptidoglycan and ends with another thin membrane. E. coli is a bacillus or rod shaped, chemoheterotrophic bacteria cell that respires with anaerobic respiration using inorganic materials. E. coli is also a prokaryotic cell that reproduces asexually through binary fission for reproduction instead of meiosis.…

    • 695 Words
    • 3 Pages
    Good Essays
  • Better Essays

    In vitro studies show that tea tree oil can be used as a topical methicillin-resistant Staphylococcus aureus (MRSA) eradication regimen in a laboratory setting. This report has been researched using a wide range of journals and internet sources to find out whether tea tree oil can be used as an alternative to Mupirocin for those suffering from MRSA colonisation. The journals that were searched contained useful information about tea tree oil as an antiseptic, however, many of the studies, like Hada et al (2001), looked at inhibiting the MRSA colonization rather than eradicating it. There were three studies found to be useful in this review. The first was Dryden et al (2004) which found Mupirocin to be more effective than tea tree oil by only a small percentage. The second study by Caelli et al (2001), which had considerably fewer participants, showed a large but non-significant improvement in eradicating MRSA compared to traditional treatment. Finally, the third study by May et al (2000) was performed with two chemically different tea tree oils and found a rapid killing time (less than 60 min) was achieved with both tea tree oils with most isolates, but MRSA was killed more slowly than other organisms. This study does not include murpirocin, however, it shows the success rates of tea tree oil as an antiseptic. All of these studies are cited in a review found by Flaxman (2007) which compares two randomized controlled trials which was undertaken to show the effects of tea tree oil and mupirocin in eradicating MRSA. There are currently an insufficient amount of studies to support the viability of tea tree oil in clinical practice against the eradication of MRSA.…

    • 1153 Words
    • 5 Pages
    Better Essays
  • Better Essays

    Essential oils are nearly always isolated by steam distillation, in which steam forced through the plant material vaporizes the essential oil which is then condenses into a receiver along with water from the condensed steam. Clove oil was separated from the distillate by extraction with dichloromethane, and its major component was then separated from minor components by extraction with aqueous sodium hydroxide.…

    • 1291 Words
    • 6 Pages
    Better Essays