Ionic Acid Synthesis Lab Report

2. Procedure
2.1: Experimental Prep and Optimum wavelength Selection
An ionic Iron solution with a known concentration of 4.047x10-3 M was obtained. A 250-mL sample of a 10-fold dilution of this ionic iron solution and 5 drops of concentrated HNO3 was made. This solution served as the source of ionic Iron for the remainder of the lab and was labeled “stock ionic Iron solution.” Next, a 50-mL aqueous ionic Iron and FerroZine® complex solution was prepared by adding 5.00mL stock ionic Iron, 3-mL of acetic acid buffer, 2-mL of 5% hydroxylamine hydrochloride, allowing five minutes for hydroxylamine to reduce Fe3+ to Fe2+, adding 2.5-mL of 0.01 M FerroZine® solution, and mixing well. A single beam Agilent technologies CARY60-UV-Vis Spectrophotometer was then employed to determine the optimum wavelength in the visible spectrum of the ionic Iron and FerroZine® complex.
1.2: Calibration Curve of ionic Iron and FerroZine® complex
A calibration curve for the ionic Iron and FerroZine® complex solution with absorbance values ranging from 0.1 to 1.5 absorbance units was made using the aforementioned concentrations of stock ionic Iron. This was done with an Agilent technologies CARY60-UV-Vis spectrophotometer with an optimum wavelength of 562