Iodometric: Vitamin C and Commercial Fruit Juices

Topics: Vitamin C, Iodine, Ascorbic acid Pages: 22 (3511 words) Published: January 21, 2014
ANALYSIS OF VITAMIN C IN COMMERCIAL FRUIT JUICES BY
IODOMETRIC TITRATION.

SHAMSUL AZRIN BIN MD. KANAFE

Final Year Project Report Submitted in Partial Fulfilment of the Requirement for the Degree of Bachelor of Science (Hons.) Chemistry in the Faculty of Applied Sciences,
Universiti Teknologi MARA.

APRIL 2009

This Final Year Project Report entitled “ANALYSIS OF VITAMIN C IN COMMERCIAL FRUIT JUICES BY IODOMETRIC TITRATION” was submitted by Shamsul Azrin Bin Md. Kanafe, in partial fulfillment of the requirements for the Degree of Bachelor of Science (Hons.) Chemistry, in Faculty of Applied Sciences, and approved by

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ACKNOWLEDGEMENTS

First of all, I would like to ALLAH s.w.t. for giving the strength to face life as a student in the Faculty of Applied Sciences in University Teknologi MARA. Thanks to my supervisor, Pn Mashita Abdullah @ Mohd Noor, for her perpetual energy and enthusiasm in suiding me to complete my project. My gratitude also goes to my family for their unconditional love and support throughout my life. My friends who have helped directly or indirectly during my student in the university.

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TABLE OF CONTENTS.

Page.
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ACKNOWLEDGEMENTS.
TABLE OF CONTENTS.
LIST OF TABLES.
LIST OF FIGURES.
LIST OF ABBREVIATIONS
ABSTRACT.
ABSTRAK.

CHAPTER 1 INTRODUCTION.
1.1
Background.
1.2
Significant of study.
1.3
Objectives of study.

1
3
3

CHAPTER 2 LITERATURE REVIEW.
2.1
Vitamin C
2.2
Methods to determine vitamin C.
2.2.1
Direct titration.
2.2.2
High performance liquid chromatography (HPLC).
2.2.3
Enzymatic method.

4
4
4
6
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CHAPTER 3 METHODOLOGY.
3.1
Method using iodometric titration
3.2
Materials and reagents.
3.3
Preparation of iodine solution.
3.4
Preparation of vitamin C standard solution.
3.5
Standardisation of iodine solution with the vitamin C standard solution 3.6
Titration of juice samples.

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CHAPTER 4 RESULT AND DISCUSSION

10

CHAPTER 5 CONCLUSION AND RECOMMENDATION

15

CITED REFERENCES
APPENDICES
CURRICULUM VITAL

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17
18

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LIST OF TABLE.

Table
4.1
4.2

Caption
Table amount of vitamin C in fruit juice samples analysed.
Comparing amount of vitamin C in nature fruit juices and commercial fruit juices.

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LIST OF FIGURES.

Figure
1.0
4.1

Caption
The structure of vitamin C or ascorbic acid
The amount of vitamin C for three commercial fruit juices analysed

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LIST OF ABBEREVIATIONS.

Ascorbic acid

:

Vitamin C

D-IAA

:

D-isoascorbic acid

HPLC

:

High pressure liquid chromatography.

L-AA

:

L-ascorbic acid.

Shelf life before expired

:

Period between experimental date and expired date

UV-VIS

:

Ultraviolet-visible.

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ABSTRACT.
ANALYSIS VITAMIN C CONTENT IN COMMERCIAL FRUIT JUICE
SAMPLES SUCH AS APPLE JUICE, GUAVA JUICE AND MANGO JUICE.

The objective of study are determination vitamin C content in three commercial fruit juices (apple, guava and mango) using titration method. Beside that, comparing vitamin C content determined and to that stated on the label for each fruit juice sample. To relate the amount of vitamin C present at the time of consumption based on the expired date. Sample to determine vitamin C is apple juice, mango juice and guava juice in commercial fruit juice on different expiry date. From different expiry date can get different shelf life before expired days. Method to determine vitamin C in commercial fruit juice is titration method with iodine solution. Titration method is accurate and precision method compare another methods. From the result the higher amount of vitamin C was guava juice about 183 g/100mL for nature fruit and 20.25 g/100mL for commercial fruit juice. Following mango juice was...
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