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Iodine Test Lab

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Iodine Test Lab
Josh Huggard
Mr. Neale SBI3U1
November 6th, 2014
Spit and Armpit Lab
Partners: Kara Washer and Josh Young

Abstract

This lab shows the use of salivary amylase with strong and weak starch mixtures to break down complex carbohydrates into simpler sugars. This lab was conducted to physically see the breakdown of carbohydrates into simpler sugars (glucose, fructose, galactose) using the salivary amylase enzyme. This is extremely important to all metabolic functions in the human digestive system. It is found that benedict’s solution, when heated makes a yellow-orange colour to indicate the simple sugars. Iodine is the indicator of a complex carbohydrate.Without enzymes that help these metabolic events absorption during digestion would be without
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The Iodine turned black indicating carbohydrates were present after being added to the strong starch solution.
2
Strong starch and Benedicts Soloution (heated)

The test tube was heated for 5-6 minutes. After 2 minutes of making sure it was nothing was going to change after heating, it was clear that there was no change had occurred with the benedict’s solution. The benedicts solution stayed a light blue color when added to the strong starch solution, and continued to stay the same color after heated. This indicates no simple sugars present.
3
Weak starch, saliva, iodine

The test tube was held underneath the armpit for 10-11 minutes. Once the iodine was added and 2 minutes had passed, it was clear that no change would come to the iodine soloution. The iodine continued to remain a rust colour after being added to the weak starch and saliva soloution after being heated underneath the armpit. This indicates complex carbs were not present
4
Weak starch, salive, benedicts soloution
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Saliva itself is used to moisten and lubricate the mouth, and wet food to help create a bolus so that the food is easier to swallow. Both saliva and salivary amylase are produced by the salivary glands, which are found underneath the lower jaw, underneath the tongue and beside the ears.

Once starch is in a mouth, salivary amylase will digest the starch in food to maltose, which will later get digested to glucose in the Duodenum. This is the beginning of chemical digestion of carbohydrates. Salivary amylase also helps to keep the pH in the mouth basic before entering the stomach, where it will turn acidic.

There are many elements that are necessary to the survival of all living things. Enzymes are one of these. Enzymes are the foundation of energy and also the life force in all living things. They are vital in building, detoxifying, and healing the body. They are also the force that allows your body to digest and absorb food. Enzymes are also very important because they regulate tens of thousands of other biological functions that occur in the body on a daily basis. Even basic thought will involve

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