Investigating the Effect of Enzyme Concentration on the Rate of an Enzyme-Catalysed Reaction.

Topics: Potato, Oxygen, Measurement Pages: 3 (671 words) Published: December 1, 2008
Aim:
This experiment will aim to show how the amount of substrate affects the rate of the reaction. Hypothesis:
In this experiment I think the amount of substrate will simply increase the reaction. As I increase the surface area of the potato the gas given off from the reaction will increase. Therefore to sum things up, my hypothesis is when the Independent variable increases so will the dependant variable. Independent variable:

-Surface area of potato
Dependant variable:
-Gas
Controlled variable:
-Equal size of potatoes used
-Using measuring cylinder
Equipment used:
-Gas collecting kit
-Potato
-Stop watch
-Test tube
-Distilled water
-Hydrogen peroxide
-Knife
Safety precautions:
Dealing with corrosive chemicals “hydrogen peroxide”
Method
1.Cut potatoes into 9 equal pieces.
2.Peel off skin.
3.Cut each potato chip into ascending order.
4.Add buffer of 4.8 ml.
5.Add 1.4 ml of hydrogen peroxide
6.Add potato chip & close bung.
7.Take measurement of gas after 2 minutes.
8.Repeat.

Investigating the effect of enzyme concentration on the rate of an enzyme-catalysed reaction.

James Moore


Conclusion:
For the conclusion we see that in fact the hypothesis is correct. When independent variable increases so does the dependent variable. As the amount of enzyme concentration increases so will the rate of the enzyme catalysed reaction. In the human body substrate is always available therefore there is only one possibility and that a constant increase in the rate of reaction. When there is higher enzyme concentration there will be more active sites available so therefore more oxygen will be given off from the hydrogen peroxide. Reaction occurs in the active site therefore it is evident that as enzyme concentration increases so will the rate of the reaction. This is proved by the graph as we can see that it does not plateau but the rate of reaction continues to increase no matter what. As we can see from the table of...
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