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iLab week 7

By Heem2006 Sep 12, 2014 298 Words
CHEM120 Week 7 iLab: DNA, mRNA, and Protein (30 points)

Complete the two questions below. Each question has four parts. This assignment is two pages long.

Question 1:

For the following double-stranded DNA sequence,
-CATTGACCGTAA-
-GTAACTGGCATT-

Answer the following questions:

a) Assume that RNA polymerase will read the top strand of DNA as the “template” to synthesize mRNA. What will be the sequence of the mRNA synthesized? (3 points) The new mRNA sequence will be GUAACUGGCAUU

b) Based upon the mRNA sequence above, what is the peptide sequence? (4 points)

The peptide sequence will be GUA-ACU-GGU-AUU, which translates to VAL-THR-GLY-ILE

If there was a mutation in the DNA sequence above resulting in the following DNA sequence, -GATTGACCGTAA-
-CTAACTGGCATT-

c) What would be the resulting mRNA, assuming RNA polymerase will use the DNA sequence, -GATTGACCGTAA- ? (4 points) The new mRNA sequence will be CUAACUGGCAUU.

d) Based upon the mRNA sequence above, what is the peptide sequence? (4 points)

The peptide sequence will be CUA-ACU-GGC-AUU, which will translate to LEU-THR-GLY-ILE.

Question 2:

For the following DNA sequence,
-CCCTTAGATGGA-
-GGGAATCTACCT-

Answer the following questions:

a) Assume that RNA polymerase will read the top strand of DNA as the “template” to synthesize mRNA. What will be the sequence of the mRNA synthesized? (3 points)

The new MRNA sequence will be GGGAAUCUACCU

b) Based upon the mRNA sequence above, what is the peptide sequence? (4 points)

The peptide sequence will be GGG-AAU-CUA-CCU, which translates to GLY-ASN-LEU-PRO.

If there was a mutation in the DNA sequence above resulting in the following DNA sequence, -CTCTTAGATGGA-
-GAGAATCTACCT-

c) What would be the resulting mRNA, assuming RNA polymerase will use the DNA sequence, - CTCTTAGATGGA - ? (4 points)

The new MRNA sequence will be GAGAAUCUACCU.

d) Based upon the mRNA sequence above, what is the peptide sequence? (4 points)

The peptide sequence will be GAG-AAU-CUA-CCU, which translates to GLU-ASN-LEU-PRO.

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