Preview

Identifying Salmonella Choleraesuis

Powerful Essays
Open Document
Open Document
1410 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Identifying Salmonella Choleraesuis
Jessica Beckman
Bio 351 - General Microbiology
Concordia University, Irvine
February 24, 2012
Identifying Salmonella choleraesuis using Several Microbiology Lab Techniques The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test, an oxidase test, a gram stain, a hanging drop slide, and morphology identification. The unknown bacterium, which was contaminated with Serratia marcescens, was isolated by streaking the bacteria solution to single colonies. The isolated unknown white bacteria, had the appearance of circular form, convex elevation, entire margin, elongated cocci. The tests than showed that the bacteria was gram-negative, non-motile, and was identified as a Salmonella species bacteria, Salmonella choleraesuis.

Introduction When applying biology to real world cases, it takes numerous tests to be able to identify exactly what a type of bacteria an unidentified bacterium is, whether it is a bacterium that is causing a person to be ill or a bacterium that is being studied in a laboratory. Often times a bacteria is taken from a contaminated sample that may contain more than one type of bacteria and therefore the specific bacteria that is to be identified has to be isolated. This can often be done by streaking the sample to single colonies. The individual colonies can then be put through several different tests that allow one to identify distinctive aspects of the bacteria. Some examples of these tests are a gram-stain and an API test. These tests allow one to figure out what properties a bacteria strain contains, such as gram negative or gram positive with the gram staining test, or what bacteria an unknown is based on positives and negative of the API test. The results of API tests, will show extremely accurately what a bacteria is based on previous tests performed. One such bacteria than can be identified is Salmonella choleraesuis. There have been many



References: Agbor, T. A., & McCormick, B. A. (2011). Salmonella effectors: Imortant players modulating host cell function during infection. Cell Microbiology , 13 (12), 1858-69. Schwarz, P., Kich, J. D., Kolb, J., & Cardoso, M. (2011). Use of an avirulentlive Salmonella Choleraesuis vaccine to reduce the prevalence of Salmonella carrier pigs at slaughter. Vet Rec , 21 (169), 553.

You May Also Find These Documents Helpful

  • Better Essays

    The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria.…

    • 1118 Words
    • 5 Pages
    Better Essays
  • Powerful Essays

    In todays world of ever evolving genetic mutations the incidence of drug resistant forms of microorganisms are on the rise. The importance of identifying these pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course, students were to determine the identity of an assigned unknown organism. Observations were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables, charts and drawings herein and reflect the results of microscopic observations as well as the differential tests results on various agars and broth cultures. Although all tests were not conclusive, the unknown organism labeled Unknown #11 was found to be a member of the family Enterobacteriacea and Genus Serratia marcescens.…

    • 1279 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    As mentioned in Exercise 8, “Identifying Gram Negative Rods”, identifying bacteria is a common activity in the microbiology lab. Like the game Clue™, each time you gather a piece of information to solve the mystery, you gather some information that supports some identities and eliminates others from contention. In the lab, the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus, identification of microbes is a process of elimination based on logic and carefully-performed tests to determine the capabilities of the unknown microbe.…

    • 2934 Words
    • 11 Pages
    Good Essays
  • Good Essays

    Salmonella

    • 877 Words
    • 4 Pages

    As their name suggests Salmonella enterica are involved in causing diseases of the intestines (enteric means pertaining to the intestine). The three main serovars of Salmonella enterica are Typhimurium, Enteritidis, and Typhi. Each of these is discussed further below. These distinctions are are designed to help scientists distinguish similar bacteria from each other in papers and when discussing the genetics.…

    • 877 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Salmonella Essay

    • 724 Words
    • 3 Pages

    There are many things in the world that can make a person sick. A lot of times, it is a simple matter of just being careless, or ignorant of the facts, that causes a person to get sick from the foods or animals that they handle. One type of bacteria that can be picked up from these bad practices is Salmonella. If left unchecked, or if action isn’t taken, then this bacteria has the potential to develop into an illness, spread to other people, and possibly be the cause of an outbreak.…

    • 724 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Salmonella Case Study

    • 818 Words
    • 4 Pages

    Normal symptoms of salmonella can be diarrhea, fever, nausea or abdominal cramps. The only way to determine 100% if you are ill due to salmonella is with laboratory tests. Most people that do become ill with salmonella do not need treatment because most cases clear up on their own after 5-7 days. If your condition worsens or you happen to still feel sick after that time period some antibiotics may be needed to clear out the salmonella. During this time is it very important to stay hydrated with…

    • 818 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Since mid- 1990´s a number of reports have been published on the isolation of different Salmonella strains, similar to S. Typhimurium but a difference in the flagella antigen, in several European countries as well as in the U.S.A (EFSA Journal 2010, Andrea et al. 2008). As known, serotyping of Salmonellas is based on antigenic variability of LPS (O Antigen), flagella (H antigen) and capsule (VI antigen). Most Salmonella strains are motile by means of flagella, the flagella can be encoded by 2 genes (fljB, fljC), which expression is regulated through a mechanism called “Phase variation”. Most of the salmonella strains express both phases and are therefore called “biphasic” (Heather R et al. 2003). In S. Typhimurium strains antigen H has 2 phases, one is called “i” and the second “1,2”. Salmonella strains with antigenic structure similar to that of S. Typhimurium but lacking certain flagellar antigens phases are called ‘Salmonella Typhimurium like’.…

    • 957 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Salmonella

    • 1150 Words
    • 5 Pages

    The unknown organism (#11) was Salmonella enterica, which is a rod-shaped, Gram negative organism. They are non-spore forming; very motile .These organisms are chemoorganotrophs (an organism that obtains energy from the oxidation of reduced organic compounds). Salmonella enterica makes ATP by aerobic respiration if oxygen is present but also can switch to fermentation in the absence of oxygen (facultative anaerobes). Salmonella bacteria do not maintain the crystal violet dye used in the gram staining method because of their cell walls that are thin .most of the gram negative bacteria are usually harmful to the host. (Gram negative rod shape salmonella entrica)…

    • 1150 Words
    • 5 Pages
    Good Essays
  • Better Essays

    Cholera

    • 1424 Words
    • 6 Pages

    8) Joachim R, Karl EK (2002) Vibrio cholerae and cholera: out of the water and into the host. FEMS Microbiology Reviews 26, No.2, Pp.125-139.…

    • 1424 Words
    • 6 Pages
    Better Essays
  • Powerful Essays

    With the alarming increase of health threatening diseases caused by microbial organisms in the world, Philippines is one of the countries that has a rapid spread of different systematic bacterial diseases. Number of victims near Manila has risen to 1,447 as of February 2008 (Entis, 2008). Some of these contaminations are caused by Salmonella spp., a member of the family Enterobacteriacea, a fairly common cause of food poisoning (Burton and Engelkirk, 2004).…

    • 3954 Words
    • 16 Pages
    Powerful Essays
  • Good Essays

    Project Title: Examination of bacterial content of sea water samples from selected sites in South East England Introduction…

    • 1418 Words
    • 6 Pages
    Good Essays
  • Better Essays

    Salmonella Enterica

    • 1413 Words
    • 6 Pages

    Salmonella is still a very common source of foodborne illness! As of January 14th 2014 there was a reported outbreak of Salmonella Enterica Heidelberg involving Tyson mechanically separated chicken, which has (so far) infected 9 individuals (CDC, n.d.). What is Salmonella Enterica, and how do we prevent it from spreading? Research mechanisms, virulence, prevention and treatment are all very important aspects of a bacterial infection and each topic will be thoroughly discussed in the text below.…

    • 1413 Words
    • 6 Pages
    Better Essays
  • Good Essays

    lab report

    • 2100 Words
    • 9 Pages

    INTRODUCTION There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose, Indole Production, and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous in his stool. The patient had no pain but mild discomfort during bowel movements. The doctor noted inflammation of the lining of the rectum. The doctor removed a tissue sample for examination and referred the patient to a gastroenterologist for a barium xray and colonoscopy. While utilizing the methods stated above, the bacterium found was a facultative anaerobe due to the Gram Negative Rods, fermentation, and the production of indole. The specific Gram Negative Enteric Bacilli was Shigella dysenteriae. There are many reasons for knowing how to control bacterial growth as well as such as antimicrobial drugs, which assist the body’s defenses against pathogens. Microorganisms are capable of causing infection or spoilage. Controlling bacterial growth is important in poultry farm businesses that use equipment to decontaminate processing plant fluids before disposal. During quality control and safety evaluations, bacteria growth that needs to be controlled can be found. While controlling bacterial growth, clusters of Gram Positive Cocci were found. It was found some ways to control bacterial growth were disinfection, sterilization, antisepsis, sanitization, moist and dry heat, filtration, and radiation; just to name a few. The specific method used for control depends on various circumstances.…

    • 2100 Words
    • 9 Pages
    Good Essays
  • Better Essays

    Many infectious diseases caused by infectious agents, including bacteria, fungi, viruses and parasites, have plagued human existence CITATION Kon97 \l 1033 (Koneman, 1997). Thus the identification of bacteria becomes all the more important in the search for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars, various other tests need to be done to differentiate and confirm their identity.…

    • 2005 Words
    • 8 Pages
    Better Essays
  • Powerful Essays

    The New Year's Eve Crisis

    • 1626 Words
    • 7 Pages

    Number of salmonella-related deaths and age-adjusted mortality rates per 100,000 population by year, United States, 1991-2000…

    • 1626 Words
    • 7 Pages
    Powerful Essays