HW10 9 14 The Cell Cycle And Cancer Worksheet 1

Name: _Amanda Lonczynski____________________ Date:_3/31/15____________

Instructions:

1. Go to: www.mhhe.com/tlw7, Click on “student edition” and go to “Virtual Labs”
2. Click on link: “The Cell Cycle and Cancer”. This will open a new window/tab.
3. Download instructions and Lab Report and Post Lab Quiz
4. Complete Laboratory Exercise
5. Answer Lab Report and Post-Lab Quiz (below for your convenience).

Virtual Lab: The Cell Cycle and Cancer
Worksheet

1. In which phase of mitosis do each of the following occur:
a. Centromeres split and chromosomes move toward opposite sides of the cell
b. Chromatin coils to form visible chromosomes
c. The nuclear membrane disappears
d. Sister chromatids line up in the center of the cell

2. In which phases of mitosis are sister chromatids visible, and attached to each other at the centromere?

Table 1: Record your data for the number of cells in each stage of the cell cycle observed in normal tissues.

Tissue Type
# Cells in Interphase
# Cells in Prophase
# Cells in
Metaphase
# Cells in Anaphase
# Cells in
Telophase
Lung Tissue
Sample 1
19
0
0
1
0
Lung Tissue
Sample 2
19
1
0
0
0
Stomach Tissue
Sample 1
16
0
1
0
2
Stomach Tissue
Sample 2
19
0
0
1
0
Ovarian Tissue
Sample 1
18
0
1
2
0
Ovarian Tissue
Sample 2
19
0
0
1
0

Table 2: Record your data for the number of cells in each stage of the cell cycle observed in cancerous tissues.

Tissue Type
# Cells in Interphase
# Cells in Prophase
# Cells in
Metaphase
# Cells in Anaphase
# Cells in
Telophase
Lung Tissue
Sample 1
15
1
3
0
1
Lung Tissue
Sample 2
16
0
2
1
1
Stomach Tissue
Sample 1
13
2
2
2
1
Stomach Tissue
Sample 2
14
2
1
1
2
Ovarian Tissue
Sample 1
11
2
2
3
2
Ovarian Tissue
Sample 2
12
2
1
2
3

Table 3: Use the data in Table 1 to calculate the Mitotic Index (average % cells dividing) for each normal tissue type.

Tissue Type
Avg. % cells at rest
Mitotic Index
Lung - normal
95.00%
5.00%
Stomach - normal
88.00%
10.00%
Ovary - normal
93.00%
10.00%