Hsc2 Unit 1 Assignment
MOHAWK COLLEGE OF APPLIED ARTS AND TECHNOLOGY | Genetics – HSCI 10022 | Assignment # 1 | | | |
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Assignment Due Date: Tuesday, March 8th 2011
Prepared By:Jelena Komazec Student Number:000012828
Prepared For: Dr. U. Vivegananthan
i) The enzyme that would be used to cut the vector would be SalI along with adding the CIP. The main reason that you would cut with the particular enzyme Sal I is because the beginning of the vector sequence there isn’t a stop codon, so but cutting it with Sal I than both the vector and the protein will be compatible. There however is going to be a function of the CIP. CIP is a very important aspect of the whole process because once the vector is opened; the CIP is going to
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Assignment Due Date: Tuesday, March 8th 2011
Prepared By:Jelena Komazec Student Number:000012828
Prepared For: Dr. U. Vivegananthan
i) The enzyme that would be used to cut the vector would be SalI along with adding the CIP. The main reason that you would cut with the particular enzyme Sal I is because the beginning of the vector sequence there isn’t a stop codon, so but cutting it with Sal I than both the vector and the protein will be compatible. There however is going to be a function of the CIP. CIP is a very important aspect of the whole process because once the vector is opened; the CIP is going to