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How Does Temperature Affect The Rate Of An Enzyme Reaction

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How Does Temperature Affect The Rate Of An Enzyme Reaction
Introduction

“The Enzyme Reaction” An enzyme is a protein that acts as a catalyst, which brings out a biochemical reaction. A Catalase enzyme, the enzyme tested in this experiment, is found in almost all living organisms that are exposed daily to oxygen (such as fruits, vegetables and animals).

Background Information
The Catalase enzyme in this experiment is known for being less affective the warmer the temperature is. According to “Science fair projects” an enzyme becomes unstable at higher temperatures and the shape of the enzyme changes. The enzyme is also used to remove hydrogen peroxide from clothing in the textile industry. This would make sense because when an enzyme encounters a substrate molecule, the interaction between the enzyme and the substrate is weak when broken apart, resulting in a different shape to strengthen the binding. An example of this complex is shown below:
The Catalase enzyme is also used for commercial use, not only playing the role of life in a living organism. The enzyme is used to remove hydrogen peroxide from milk in order for milk to be used in making cheese. The enzyme is also used to remove hydrogen peroxide from clothing in the textile industry.
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A potato will be the substance used to determine how different temperatures affect this enzyme. This experiment has a control, independent, and dependent variable. The control variable is the size of the filter paper, the amount of potato in the experiment, and the amount of hydrogen peroxide used. The independent variable is the hydrogen peroxide and the potato’s temperature. The dependent variable is the reaction time in the test tube. My hypothesis is that I predict temperatures above 30 degrees Celsius the enzyme will not be as

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