Histology Lab

Topics: Muscle, Epithelium, Cardiac muscle Pages: 9 (1341 words) Published: February 26, 2014


Histology
Laszlo Vass, Ed.D. Version 42-0013-00-01

Lab RepoRt assistant
This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Purpose

What is the purpose of this exercise?
The purpose of this exercise is to use the microscope to get an in depth look at different cell structures and to learn how the shape and arrangement of different cells can affect their functions.

Are there any safety concerns associated with this exercise? If so, list what they are and what precautions should be taken. Some safety concerns could be while assembling and moving the microscope it is important to be careful as to not break the microscope. It is a very delicate piece of equipment and should always be handled with two hands when being moved and should always be on a flat surface.

Exercise 1: Epithelial Tissue

Data Table 1: Epithelial Tissue Observations
TISSUE TYPE
OBSERVATIONS

Simple Squamous
 Flat, scale-like cells, and very close together.

Simple Cuboidal
Single layer of cube/oval like cells, cells are not packed together

Simple Columnar (stomach)
Single layer of different sized tall, narrow cells, connective tissue

Simple Columnar (duodenum)
Columnar cells, brush border, basement membrane, goblet cells, connective tissue.

Stratified Squamous (keratinized)
Multiple cell layers with cells becoming increasingly flat and scaly toward surface

Stratified Squamous (non-keratinized)
Cells long flat cell boarded by a darker layer of cells

Pseudostratified Ciliated Columnar
3 layers of cells; surface cells are round or square, one layers cells are spread out

Transitional
Surface cells are rounded and often bulge at surface, somewhat looks like stratified squamous.

Stratified Cuboidal (online)
2 distinct layers, round cells above a layer of more oval cells.

Stratified Columnar (online)
2 layers of column shaped cells, with a basal layer of cuboidal cells and looks like there is a single nucleus.

Questions

A. Why is the study of histology important in the overall understanding of anatomy and physiology? Histology is important to the overall understanding of anatomy and physiology because it gives us an up close look at the structure and composition (anatomy) of our tissues and cells through use of a microscope, and from there we can begin to understand how they function (physiology).

B. How are epithelial tissues named?
Epithelial tissues are named based on their size and shapes of cells, for example cuboidal are cube shaped, columnar are column shaped.

C. Why are some epithelial tissues stratified?
Some epithelial tissues are stratified because they have multiple layers of cells that function as protection.

D. Unlike squamous cells, cuboidal and columnar cells have large, open cytoplasm. Which functions of epithelial tissue are supported by having such big cells?

E. Look at the following drawings and identify each type of epithelial tissue:

1. Simple Squamous

2. simple columnar

3. Pseudo stratified columnar

4. Stratified Squamous

Exercise 2: Connective Tissue
obseRvations

Data Table 2: Connective Tissue

Tissue
Amount and Shape of
Cells
Amount of
Matrix
Are there fibers? If so,
are they parallel or
scattered?
Mesenchyme (online)
23-polygonal
20%
Both

Aerolar (online)
28-Spindal
30%
Parallel
Adipose (online)
8-Polygonal...
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