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CHAPTER III
METHODOLOGY
MATERIALS, METHODS AND TREATMENTS
Gathering and Preparation of Materials In the present study, the Researchers chose to use Rattus norvegicus (Sprague Dawley Rats) since rats are considered one of the most commonly used animals in the laboratory. Rat strain is created through breeding to produce rats that are similar to each other and can therefore be used in research. Wistar, Fisher and Sprague Dawley, and their hundreds of internal divisions, are examples of rat strains. Most laboratory rat strains descend from a colony of rats established at the Wistar institute in 1906. The Sprague Dawley strain came from crosses between Wistar Females and a “hybrid” male whose origins are unknown. Long-Evans rats are thought to come from crosses between Wistar Females and a wild Norway Rat. (Rat Behavior 2003-2004) The researchers plan to use Sprague Dawley rats in their experiment in order for them to get enough amount of blood to be used for laboratory testing. The researchers refrain from using mice since the blood that they can obtain is less compared when they use rats. In addition, the researchers think that the mice might suffer or worst die if too much blood would be gathered from them. The researchers also refrain from using guinea pigs and rabbits since both of them do not have tails. The tails part is the region wherein the bloods being gathered. Rats were the animals that were the only ones available and that are very cost effective. Moreover, there are necessary details that are within the rats only. They have long tails and that they are big enough that’s why they wouldn’t suffer too much if blood would be obtained from them. Lastly, their DNA characteristics are close to that of humans. (Sprague Dawley Rats, 2011) A total number
METHODOLOGY
MATERIALS, METHODS AND TREATMENTS
Gathering and Preparation of Materials In the present study, the Researchers chose to use Rattus norvegicus (Sprague Dawley Rats) since rats are considered one of the most commonly used animals in the laboratory. Rat strain is created through breeding to produce rats that are similar to each other and can therefore be used in research. Wistar, Fisher and Sprague Dawley, and their hundreds of internal divisions, are examples of rat strains. Most laboratory rat strains descend from a colony of rats established at the Wistar institute in 1906. The Sprague Dawley strain came from crosses between Wistar Females and a “hybrid” male whose origins are unknown. Long-Evans rats are thought to come from crosses between Wistar Females and a wild Norway Rat. (Rat Behavior 2003-2004) The researchers plan to use Sprague Dawley rats in their experiment in order for them to get enough amount of blood to be used for laboratory testing. The researchers refrain from using mice since the blood that they can obtain is less compared when they use rats. In addition, the researchers think that the mice might suffer or worst die if too much blood would be gathered from them. The researchers also refrain from using guinea pigs and rabbits since both of them do not have tails. The tails part is the region wherein the bloods being gathered. Rats were the animals that were the only ones available and that are very cost effective. Moreover, there are necessary details that are within the rats only. They have long tails and that they are big enough that’s why they wouldn’t suffer too much if blood would be obtained from them. Lastly, their DNA characteristics are close to that of humans. (Sprague Dawley Rats, 2011) A total number