Group Partners: Jenny Case
Alexander Davis
Period: 1 Group Partners: Jenny Case Blake Rebek Matthew Heigm Christian Hogdohm
Gel Electrophoresis
I. Introduction: A typical electrophoresis has five major parts: the electrical current, DNA, RNA, or protein sample, …show more content…
The E-Gel was removed from the package and put it into the power base, right edge first. The E-gel is then firmly seated or “snapped” into the power base. A red light confirms this. 3. The E-Gels are pre-run for 2 minutes before we loaded the samples. A flashing green light confirms this stage has started. 4. At the end of the pre-run phase the current stops running through the E-Gel and the flashing green light changes to a flashing red light and a beeping sound is heard. 5. The comb in the E-Gel is removed by carefully pulling it straight up. 6. Using a micropipette, a 10 micro liter sample is dispensed into well by holding the tip of the micropipette just inside the well so as not to puncture the bottom. 7. We continued inserting the remaining dye samples into each of the remaining wells, using the same amounts for each well. For example: Well #2 contains Dye sample #2 and Well #3 contains Dye sample#3 and so on. 8. After we loaded the E-Gel, we started the 35-minute electrophoresis run. This was confirmed by a steady green light. 9. The current automatically runs for 35 minutes. This is confirmed by a Flashing red light a beeping sound. Turning it off stops the beeping sound. 10. The E-Gel if then removed from the Power Base. The gel is …show more content…
Results(Analysis Questions):
Dyes: #4, #6, and #10 traveled the fastest because they contained a protein with a molecular weight that was smaller than the others. The dye mixtures contained a sample of DNA, RNA or other protein. These were negatively charged so that they traveled toward the cathode. There were twelve samples in all, labeled #1-#12. Agarose gel--It provides a medium for the dye to travel through.
Running Buffer- A liquid that separates the proteins in a gel matrix.
Wells in gel- The entry point for the dye.
Electric Current- It softens the gel and provides the energy for the process to take place.
A strand of DNA is negatively charged and moves through the agarose gel when an electric current is applied. Electrophoresis sorts different-sized molecules according to their size. Smaller sized molecules travel farther toward the bottom of the E-Gel and larger molecules move toward the
Period: 1 Group Partners: Jenny Case Blake Rebek Matthew Heigm Christian Hogdohm
Gel Electrophoresis
I. Introduction: A typical electrophoresis has five major parts: the electrical current, DNA, RNA, or protein sample, …show more content…
The E-Gel was removed from the package and put it into the power base, right edge first. The E-gel is then firmly seated or “snapped” into the power base. A red light confirms this. 3. The E-Gels are pre-run for 2 minutes before we loaded the samples. A flashing green light confirms this stage has started. 4. At the end of the pre-run phase the current stops running through the E-Gel and the flashing green light changes to a flashing red light and a beeping sound is heard. 5. The comb in the E-Gel is removed by carefully pulling it straight up. 6. Using a micropipette, a 10 micro liter sample is dispensed into well by holding the tip of the micropipette just inside the well so as not to puncture the bottom. 7. We continued inserting the remaining dye samples into each of the remaining wells, using the same amounts for each well. For example: Well #2 contains Dye sample #2 and Well #3 contains Dye sample#3 and so on. 8. After we loaded the E-Gel, we started the 35-minute electrophoresis run. This was confirmed by a steady green light. 9. The current automatically runs for 35 minutes. This is confirmed by a Flashing red light a beeping sound. Turning it off stops the beeping sound. 10. The E-Gel if then removed from the Power Base. The gel is …show more content…
Results(Analysis Questions):
Dyes: #4, #6, and #10 traveled the fastest because they contained a protein with a molecular weight that was smaller than the others. The dye mixtures contained a sample of DNA, RNA or other protein. These were negatively charged so that they traveled toward the cathode. There were twelve samples in all, labeled #1-#12. Agarose gel--It provides a medium for the dye to travel through.
Running Buffer- A liquid that separates the proteins in a gel matrix.
Wells in gel- The entry point for the dye.
Electric Current- It softens the gel and provides the energy for the process to take place.
A strand of DNA is negatively charged and moves through the agarose gel when an electric current is applied. Electrophoresis sorts different-sized molecules according to their size. Smaller sized molecules travel farther toward the bottom of the E-Gel and larger molecules move toward the