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Germinating Yeast Lab

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Germinating Yeast Lab
Purpose: The purpose of this lab was to gain a complete understanding around the rate of cellular respiration within multicellular organisms, also to research and understand how to use a CO2.

Background: Living systems require free energy and matter to maintain order, to reproduce, and grow. Energy deficiencies cause disruptions at the population and ecosystem levels as well. 1 mol of H2O produces 1 mol of CO2 through cellular respiration. Autotrophic organisms capture free energy from the environment through the process of photosynthesis and chemosynthesis.

Hypothesis: The germinating beans will discharge more CO2 than the peas that are not germinating.

Materials:
1250 mL respiration chamber
Vernier CO2 gas sensor
Vernier computer interface thermometer
25 non-germinating kidney beans
25, 24 hour germinating kidney beans
25, two hour warm germinating kidney beans
25, two hour cold germinating kidney beans
Paper towels

Safety Hazards: There are no need for goggles or gloves within this lab, but be aware of the choking hazard that the beans can cause.

Procedure:
Gather all materials
Prepare a 100 mL beaker of beans/peas with warm water (warm germinating beans/peas), then prepare a 2nd 100 mL beaker with cold water instead of warm water (cold germinating beans/peas)
…show more content…
Due to the control, non germinating peas/beans are releasing less CO2 ppm than the 24 hour and 48 hour germinating peas/beans. This can be seen when the control from 0-5 minutes was at -76 ppm, while the 24 hour beans were at +374 ppm. The alaskan peas also saw this same type of pattern from 0-5 minutes. The control for the peas was at -176 ppm and the germinating peas were at +1,056 ppm. Since this lab was conducted with precision, and the sensors were not broken, or tampered with before, during or after the experiment, this data is accurate which leads to precise

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