Gene Technology

Topics: DNA, Molecular biology, Enzyme Pages: 15 (2262 words) Published: August 7, 2011
010 Chapter 10 Gene Technology
Student: _________________________________________________________ 1. Trimming certain genes out of molecules of DNA requires the use of special: 

A.  digestive enzymes

B.  restriction enzymes

C.  enzymes from peroxisomes

D.  microscopic scalpels

2. To seal the cut fragments of DNA together, an enzyme called __________ is used. 

A.  amylase

B.  peptidase

C.  trypsin

D.  ligase

3. Choose the correct sequence for gene transfer procedures. 

A.  cleaving DNA, recombining DNA, cloning, screening

B.  screening, cleaving DNA, cloning, recombining DNA

C.  recombining DNA, screening, cloning, cleaving DNA

D.  cleaving DNA, cloning, screening, recombining DNA

4. The step that is most labor-intensive in gene transfer procedures is: 

A.  cleaving DNA

B.  recombining DNA

C.  screening

D.  cloning

5. Some bacteria, through natural selection, have acquired some extremely potent enzymes that destroy viral DNA, thereby preventing the bacterial cell from becoming infected with the virus. These enzymes are called: 

A.  DNA polymerases

B.  DNA ligases

C.  restriction endonucleases

D.  restriction ligases

6. When researchers wish to make multiple copies of a gene without first inserting it into a bacterium, they employ the: 

A.  centrifuge

B.  pepsin activation reaction

C.  polymerase chain reaction

D.  gel electrophoresis

7. If a fragment of eukaryotic RNA is needed that is made up only of exons, the place to look in the cell to find this is: 

A.  attached to the inner cell membrane

B.  inside the nucleolus

C.  inside the nucleus

D.  in the cytoplasm

8. Primers are: 

A.  short sequences of nucleotides on either side of the gene to be amplified

B.  sticky ends of DNA fragments

C.  beginning nucleotide sequences in plasmids

D.  types of undercoating for paint

9. The enzyme used to carry out the polymerase chain reaction on DNA is: 

A.  transcriptase

B.  reverse transcriptase

C.  RNA polymerase

D.  DNA polymerase

10. The polymerase chain reaction (PCR) is also used to: 

A.  clone entire organisms

B.  make polyploid copies of the human genome

C.  make DNA "fingerprints" in criminal investigations

D.  clone bacterial colonies

11. The enzyme used to make a DNA copy complementary to processed mRNA is: 

A.  transcriptase

B.  reverse transcriptase

C.  RNA polymerase

D.  DNA polymerase

12. DNA fingerprinting is a highly accurate method of identifying a criminal from evidence, (blood, hair, skin, semen) left at a crime scene.  True    False
13. Inserting a gene encoding a pathogenic microbe's surface protein into a harmless virus is the way a(n) _________________ is produced. 

A.  piggyback vaccine

B.  clone of antibodies

C.  virulent virus

D.  active disease-causing pathogen

14. A friend asks you a question after your biology class. "What's genetic engineering?" Your answer is: 

A.  "Genetic engineering is the ability of scientists to remove a nucleus from a cell and place it into another cell."

B.  "Genetic engineering is taking proteins from one organism and placing them into another organism of a different species."

C.  "Genetic engineering is moving genes from one organism to another."

D.  "Genetic engineering is removing structural components, for example a lysosome, from one part of a cell and placing those structures elsewhere in that cell."

15. Genetic engineering in agriculture has been employed to: 

A.  make crops resistant to insect...
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