Gatorade Synthesis Lab Report

This experiment consisted of two parts. First, the absorption of each dye was evaluated by diluting (1 mg/mL standard) 2.0 mL of dye to 100 mL. The absorbance of each dye standard was measured and documented. The absorbance of the Gatorade was then measured by diluting the solution and pipetting 5.0 mL into a 25.0 mL volumetric flask. The absorption spectrum of the chosen drink was measured. Next, the purple Gatorade extraction procedure was carried out, beginning with pipetting 1 mL of 70% isopropanol (IPA) and 1 mL of saturated salt water into test tube 1 (solution 1). Then, pipette 2 mL of saturated salt water into test tube 2 (solution 2), 2 mL of purple Gatorade into test tube 3 (solution 3), 4.0 mL of 17% IPA into test tube 4 (solution
Then, solution 2 was aspirated by mixing it with about 7 mL of air, waiting 10 seconds, and dispensing. Then, solution 3 was operated by mixing about 7 mL of air, waiting 30 seconds, and dispensing. If the solution does not appear colorless, repeat step 3. Then, solution 4 was slowly aspirated and mixed with air for approximately 5 mL before being dispensed after 10 seconds. Then, using a pipette, add 1.00 mL of DI water to the top of the DPX tip and dispense it through the DPX tip into test tube 4 to collect all of the red dye. Then, solution 5 was aspirated, mixed with approximately 7 mL of air, waited 10 seconds, and dispensed. Cuvettes were filled with the extracted dyes for UV-Vis examination. The UV-Vis absorption spectrum was recorded, and if the absorbance was "off-scale," the solution was diluted to 10.0 mL, and the absorbance was measured
For data analysis, each dye's maximum absorbance was determined and the matching wavelength was recorded. The dye's absorptivity at the maximum absorbance was determined in units of M-1cm-1 using Beer's Law and standard data. The dye in the beverage Gatorade was then computed, accounting for any dilution factor used to determine its absorbance. The dyes for the red and blue dyes were then computed in purple Gatorade. The absorption of caffeine was evaluated in the second half of this experiment by diluting a 1mg/mL standard 10-fold with 70% IPA using a 10 mL volumetric flask to create a 100 ppm standard. Then, using a 5 mL volumetric flask, dilute 1 mL of the 100 ppm standard to the desired concentration of 70% IPA. The absorbance of the diluted caffeine standard (20 ppm) was measured, and the absorbance was recorded for later calculation of absorption. To condition the DPX-WAX tip, 1 mL of 70% IPA and 1 mL of DI water were pipetted into test tube 1. Next, 2 mL of DI water was pipetted into test tube 2 (no salt was required). Then, pipette 1.0 mL of coke and 1 mL of DI water into test tube 3 (stir to remove any carbonation). Then, 3.0 mL of 70% IPA was pipetted into test tube