Franco lab report enzyme

Topics: Enzyme, PH, Fructose Pages: 4 (1154 words) Published: March 10, 2015
LABORATORY REPORT
Activity:

Enzyme Activity

Name:

Daniel Franco

Instructor:

Professor Jennifer Frere

Date:

03.08.2015

Predictions
Sucrase will have the greatest activity at
pH 6
Sucrase will have the greatest activity at
60 °C (140 °F)
Sucrase activity
decreases with increasing sucrose concentration

Materials and Methods
Effect of pH on Enzyme Activity
Dependent Variable
amount of product (glucose and fructose) produced
Independent Variable
pH
Controlled Variables
temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time

Effect of Temperature on Enzyme Activity
Dependent Variable
amount of product (glucose and fructose) produced
Independent Variable
temperature
Controlled Variables
pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time

Effect of Substrate Concentration on Enzyme Activity
Dependent Variable
amount of product (glucose and fructose) produced
Independent Variable
amount of substrate (sucrose) present
Controlled Variables
temperature, pH, sucrase + sucrose incubation time
4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. Optimal density is measured because it measures the amount of glucose and fructose produced. 5. Explain why denatured sucrase was used as a control.

Denatured sucrose was used as the control because activated sucrose was used to increase the activity. Laboratory Report/ Daniel Franco/ Enzyme Activity/ Professor Jennifer Frere/ 03.08.2015/ Page [1] of [4]

6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration.
In order to decrease enzyme activity.

Results
Table 1: Effect of pH on Sucrase Activity
1
2
3
average

ph 3
0.173
0.166
0.148
0.162

ph 4
0.329
0.310
0.322
0.320

Optical Density
ph 6
0.945
0.954...
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