Final Draft Lab 4
Effects of oligomycin, ouabain, p-Aminohippurate, sodium concentrations and potassium concentrations on active transport of the Malpighian tubules of Acheta domesticus
Key Words: Malpighian tubules, Acheta domesticus, PAH, ouabain, oligomycin, Na+, K+
Silvia Kilic
100488712
BIOL 3040U
CRN: 70041
Thursday, March 5th, 2015.
Results
The Malpighian tubules were incubated in the solutions for 15 minutes before being observed. The transport of CPR into the lumen was highest when there was no addition of oligomycin, ouabain and p-aminohippurate (PAH). The least transport occurred from the addition of ouabain and PAH (table 1). There was a strong positive correlation (R2=0.9881) between increased potassium concentration in the CPR solution and the transport of the solution into the tubule (figure 1). The least tubule transport occurred at 0mM potassium (score of 0) and the most tubule transport occurred at 10mM potassium (score of 4) (figure 1). There was a strong positive correlation (R2=0.9871) between increased sodium concentration in the CPR solution and the transport of the solution into the tubule (figure 2). The least tubule transport occurred at 0mM sodium (score of 0.083 ± 0.204) and the most tubule transport occurred at 160mM sodium (score of 3.66 ± 0.816) (figure 2).
Discussion
The experiment exemplified the effects of oligomycin, p-Aminohippurate (PAH), sodium and potassium on the active transport of chlorophenol red (CPR) into the cricket, Acheta domesticus, Malpighian tubules (MT). The study of the active transport system in crickets is useful in modeling how active transport works and how it can be affected or inhibited. This information can be transferred to the mammalian kidney that also has active transport present in order to move solutes between the tubules and capillaries.
This was a controlled experiment. The CPR solution without any added contents acted as a control. It was kept constant throughout each solution and gave a positive
Key Words: Malpighian tubules, Acheta domesticus, PAH, ouabain, oligomycin, Na+, K+
Silvia Kilic
100488712
BIOL 3040U
CRN: 70041
Thursday, March 5th, 2015.
Results
The Malpighian tubules were incubated in the solutions for 15 minutes before being observed. The transport of CPR into the lumen was highest when there was no addition of oligomycin, ouabain and p-aminohippurate (PAH). The least transport occurred from the addition of ouabain and PAH (table 1). There was a strong positive correlation (R2=0.9881) between increased potassium concentration in the CPR solution and the transport of the solution into the tubule (figure 1). The least tubule transport occurred at 0mM potassium (score of 0) and the most tubule transport occurred at 10mM potassium (score of 4) (figure 1). There was a strong positive correlation (R2=0.9871) between increased sodium concentration in the CPR solution and the transport of the solution into the tubule (figure 2). The least tubule transport occurred at 0mM sodium (score of 0.083 ± 0.204) and the most tubule transport occurred at 160mM sodium (score of 3.66 ± 0.816) (figure 2).
Discussion
The experiment exemplified the effects of oligomycin, p-Aminohippurate (PAH), sodium and potassium on the active transport of chlorophenol red (CPR) into the cricket, Acheta domesticus, Malpighian tubules (MT). The study of the active transport system in crickets is useful in modeling how active transport works and how it can be affected or inhibited. This information can be transferred to the mammalian kidney that also has active transport present in order to move solutes between the tubules and capillaries.
This was a controlled experiment. The CPR solution without any added contents acted as a control. It was kept constant throughout each solution and gave a positive
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