Fatty Acid and Test Specific Objective
I NUR 4
POLICARPIO, JOHN PAUL (A & B)
QUERUBIN, KIMBERLY (C & D)
QUILALA, REGENE (E & F1)
RAMOS, PRACCEDES (F2a & F2b)
SABALDICCA, VENZ (F2c & F2d)
INTRODUCTION Lipids are organic compounds found in living organisms that are insoluble or slightly soluble in water but soluble in non-polar organic solvents.
Lipids can be classified into four groups which are -fats, oils, and waxes, -compound lipids, -steroids, and -derived lipids.
Various experiments are done on lipids. Some tests are for saturation, presence of certain compounds, or for the different chemical reactions that lipids undergo.
Lipids may be composed of esters, amides, alcohols, cyclic, acyclic, or polycyclic structures, and may also be a combination of these.
General Objective: At the end of the experiment, students must be able to familiarize themselves with the different classes of lipids and to be able to identify each kind of lipid of lipid based on the chemical properties of its hydrolyzed products.
A. SPOTTING EFFECT
Specific Objective: Test for the presence of lipids by means of locating translucent spots/area in the filter paper.
Specific Objective: To test for the solubility of the suspected lipid-containing samples.
C. TEST FOR UNSATURATION (BROMINE WATER TEST)
Specific Objective: To test for unsaturation of lipids through a change in colour or discoloration of the bromine water.
To test for the presence of carbon-carbon double bonds.
D. ACROLEIN TEST
Specific Objective: To test for the presence of glycerol/glycerin or fats.
E. AMMONIUM MOLIBDATE TEST
F.1. EXTRACTION OF BRAIN LIPIDS
F.2. DETECTION OF BRAIN LIPIDS
A.) Molisch Test
Specific Objective: To determine the presence of carbohydrates in a lipid solution
B.) Ninhydrin Test
Specific Objective: To determine the reaction of the ninhydrin reagent to the residue B dissolved in water.
References: www.answers.yahoo.com “Lipids, what is it?”. Unknown Author. (January 18, 2010). February 14,2012. http://answers.yahoo.com/questi on/index?qid=201010040 03856AAUmexI www.answers.com Maitland, Jr Jones (1998). Organic Chemistry. W W Norton & Co Inc (Np). p. 139. ISBN 0-393- 97378- 6. Hunter JE (November 2006). "Dietary trans fatty acids: review of recent human studies and food industry responses". Lipids 41 (11): 967–92. PMID 17263298. Crystal, Gary. (2003). What is glycerin? Retrieved from: http://www.wisegeek.com/what- is-glycerin.htm on February 15, 2012. Malubay, Raymund. (n.d). Qualitative test for lipid. Retrieved from: http://www.scribd.com/doc/5136 7254/Acrolein-Test-and-Ester- Test-for-Lipids on February 12, 2012 Experiment 16 An Investigation into the Biochemical Nature of Lipids. Retrieved from: http://dwb4.unl.edu/Chem/CHEM 869K/CHEM869KLinks/domanski. cs.csi.cuny.edu/klibaner/LIPID.ht m on February 12, 2012