Extraction of Caffeine from Thea Sinensis
Extraction of Caffeine from Lipton Yellow Label Teal Leaves
Arlie Bamiano, Jealine Bernabe, Petrenne Caimbon*, Jhia Caso
Department of Biology, University of Santo Tomas
Abstract In order to extract pure caffeine from Lipton Yellow Label tea leaves, several extractions and phase transition techniques were employed to 6.5029 grams of sample. Initially, the tea leaves were boiled in water to extract tea from the leaves (Solid-Liquid Extraction). After extracting the tea, several steps of Liquid-Liquid Extraction methods were done with the use of Dichloromethane (DCM) to extract the crude caffeine from the tea and was dried using Anhydrous Sodium Sulfate which produced 0.1371grams of crude caffeine. The crude caffeine then undergone Sublimation and a percent yield of 0.0246% of pure caffeine were obtained.
Introduction Caffeine is the common name for trimethylxanthine that belongs to the family of heterocyclic compounds (known as purines) with the molecular formula (C8H10N4O2). [1] It is basically classified as an alkaloid, a type of substance produced as end products of nitrogen metabolism in plants. Its chemical structure is shown in Figure 1. Caffeine is naturally produced by plants including coffee, cocoa beans and tea. Extraction is a separation technique that selectively dissolves one or more of the mixture compounds into a suitable solvent. [2] Extraction of some solids can be performed by using the different chemical properties of different solvents. The primary solvent used in extracting the caffeine from tea leaves is water. But through the experiment, Dichloromethane (DCM) was used to extract caffeine from water because caffeine is more soluble in DCM (140g/mL) compared to water (22g/mL). In this case, dichloromethane-caffeine mixture can be separated on the basis of their difference in solubility. In this particular experiment, the tea leaves were first boiled in distilled water to separate the caffeine from the leaves. From the
Arlie Bamiano, Jealine Bernabe, Petrenne Caimbon*, Jhia Caso
Department of Biology, University of Santo Tomas
Abstract In order to extract pure caffeine from Lipton Yellow Label tea leaves, several extractions and phase transition techniques were employed to 6.5029 grams of sample. Initially, the tea leaves were boiled in water to extract tea from the leaves (Solid-Liquid Extraction). After extracting the tea, several steps of Liquid-Liquid Extraction methods were done with the use of Dichloromethane (DCM) to extract the crude caffeine from the tea and was dried using Anhydrous Sodium Sulfate which produced 0.1371grams of crude caffeine. The crude caffeine then undergone Sublimation and a percent yield of 0.0246% of pure caffeine were obtained.
Introduction Caffeine is the common name for trimethylxanthine that belongs to the family of heterocyclic compounds (known as purines) with the molecular formula (C8H10N4O2). [1] It is basically classified as an alkaloid, a type of substance produced as end products of nitrogen metabolism in plants. Its chemical structure is shown in Figure 1. Caffeine is naturally produced by plants including coffee, cocoa beans and tea. Extraction is a separation technique that selectively dissolves one or more of the mixture compounds into a suitable solvent. [2] Extraction of some solids can be performed by using the different chemical properties of different solvents. The primary solvent used in extracting the caffeine from tea leaves is water. But through the experiment, Dichloromethane (DCM) was used to extract caffeine from water because caffeine is more soluble in DCM (140g/mL) compared to water (22g/mL). In this case, dichloromethane-caffeine mixture can be separated on the basis of their difference in solubility. In this particular experiment, the tea leaves were first boiled in distilled water to separate the caffeine from the leaves. From the
References: [1] Anne Marie Helmenstine, Ph.D. (2013). Caffeine Chemistry What Is Caffeine and How Does It Work?. [ONLINE] Available at: http://chemistry.about.com/od/moleculescompounds/a/caffeine.htm. [Last Accessed 22 July 2013]. [2] Amrita Vishwa Vidyapeetham University (2013). Extraction of Caffeine from Tea. [ONLINE] Available at: http://amrita.vlab.co.in/?sub=3&brch=64&sim=169&cnt=1. [Last Accessed 22 July 2013].