Preview

Exploring enzyme lab report

Good Essays
Open Document
Open Document
1052 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Exploring enzyme lab report
Exploring Enzymes - Ground-Up Tissue Activity
Abstract
Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish, one whole and one ground up, which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined by observing a larger formation of bubbles, the visual indicator of hydrogen peroxide being broken down into a non-toxic form of hydrogen and water. This study allowed us to observe first hand catalase can perform further when the surface area is larger.
Introduction
A majority of individuals do not realize how important enzymes, a type of protein, are for life to exist. Organisms experience a continuum of chemical reactions taking place within their cells and these reactions wouldn’t happen without the presence of enzymes. Since enzymes are so important to life we should understand what affects their productivity to include: temperature, pH and the concentration of reactants. The purpose of this study is to test how the enzymes react when there is a larger surface area; hence an increase in the concentration of reactants.
In this experiment we use the enzyme catalase, which assists in the breakdown of hydrogen peroxide into water and oxygen. Hydrogen peroxide is a well-known chemical used in a variety of ways such as household cleaning, wound management and removing color from a variety of items. Hydrogen peroxide is a product of living cell reactions and is considered deadly and if not disposed of properly a cell killer. Although it sounds like doom and gloom this is where the catalase steps in, an enzyme to breakdown the hydrogen peroxide into the less toxic substances of hydrogen and water.
Hypothesis
If the surface area is increased than the

You May Also Find These Documents Helpful

  • Good Essays

    Formal Lab Report 2 Final

    • 1572 Words
    • 7 Pages

    Purpose: Cells produce toxic wastes, in this experiment hydrogen peroxide, and without some sort of molecule to break it down the cell will die, along with the organism itself. However with the aid of an enzyme, catalase, hydrogen peroxide is able to be broken down into an intermediate and thereafter harmless substances water and oxygen. The goal of this lab is to measure the reaction rate of this process in different substances such as a liver, a vegetable, and breast tissue. By using variables such a pH and temperature we are able to how the rate of reaction is altered or improved. If it has improved, the optimum has been discovered and the enzyme will create a higher reaction rate. If above the optimal points, proteins will denature and the reaction rate will remain the same. This is vital for cellular activity for if homeostasis is not reached enzymatic activity will decrease or the enzymes will simply denature and the toxicity within the cell will increase killing the cell.…

    • 1572 Words
    • 7 Pages
    Good Essays
  • Good Essays

    Catalase Lab Report

    • 563 Words
    • 3 Pages

    Next, 50ml of distilled water will be measured using a graduated cylinder. The 50g of catalase and the 50ml of distilled water will be put into a blender and blended until liquefied. The catalase solution will then be introduced to a Buchner funnel filtration system to separate the catalase extract from any solid particles left over from the blending process. This step will be repeated until all of the catalases have been blended and filtered. Next, 5 ml of hydrogen peroxide will be measured out using a graduated cylinder. It will then be poured into a small Erlenmeyer flask. This will be repeated five times, representing the five trials for each catalase treatment. Once the Erlenmeyer flask has been given 5ml of hydrogen peroxide, 10ml of the S. oleracea catalase will be measured out and poured into the Hydrogen peroxide filled flask. When the catalase and Hydrogen peroxide meet a reaction will occur and the hydrogen peroxide will be broken down into water and oxygen. The oxygen released from the reaction will be recorded by the Gas Pressure sensor. This step will be repeated five times for each necessary trial, then the next catalase extract will be…

    • 563 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Enzymes Lab Report

    • 371 Words
    • 2 Pages

    Explain in detail the procedure that you followed (including amount of substrate, enzyme etc, and the whole procedure including incubation times) (3 Points)…

    • 371 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Peroxidase Lab

    • 823 Words
    • 4 Pages

    The enzyme peroxidase has been shown to break down H2O2. Enzymes are known to increase the rate at which a chemical reaction occurs. We looked at factors that affected the breakdown of hydrogen peroxide. These effects are the different temperatures and pH levels the enzymes were placed in. We found that the optimum, or best condition, temperature for the enzymes tested was about 22 degrees Celsius. The optimum pH level for the enzyme was 7.…

    • 823 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Enzyme Lab Report

    • 492 Words
    • 2 Pages

    The results of our experiment showed the solutions in both tube 1 and tube 2 increasing in absorbency in the first eight minutes but then tube 1 continued to increase while tube 2 began to balance out. Tube 3, our blank, managed to stay at 0nm the entire twenty minutes. From this data, we can conclude that our hypothesis was supported that EDTA had a greater change in absorption over PTU.…

    • 492 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    Lab 1 again

    • 768 Words
    • 3 Pages

    Figure 1: The reaction rates of enzyme-catalysed reactions of hydrogen peroxide into diluted water depending of the varying percentage concentrations of catalase. Mean rates (±SE) of 5 separate experiments (n=5) are shown. Line of best fit (y = 0.0002x + 0.0038 R² = 0.9154) is presented using the trend line function in Excel.…

    • 768 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Catalase Experiment

    • 1088 Words
    • 5 Pages

    Catalysts are substances that increase chemical reactions while using less energy. They also remain unchanged after use, which enables it to be used repeatedly. Cells have special catalysts called enzymes, which are specialized proteins that help accelerate chemical cell reactions. (Evert, RF & Eichhorn, SE 2013). Enzymes also control plant metabolic processes such as respiration (Evert RF, Eichhorn SE & Perry JB 2013). This experiment focuses on the enzyme catalase. Catalase breaks down hydrogen peroxide into water and oxygen. Hydrogen peroxide is a waste product of cell metabolism that can be toxic to the cell (Evert RF, Eichhorn SE & Perry JB 2013).…

    • 1088 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Enzyme Lab Report

    • 1528 Words
    • 5 Pages

    catalase works to break down hydrogen peroxide. If the pH, temperature, or enzyme concentration changes,…

    • 1528 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Lab 6 enzymes

    • 1000 Words
    • 5 Pages

    Preparation Step 1: Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced.…

    • 1000 Words
    • 5 Pages
    Good Essays
  • Better Essays

    Enzyme Lab Report

    • 955 Words
    • 4 Pages

    Enzyme B produced the most maltose at a high temperature (100 ºC) and an acidic pH.…

    • 955 Words
    • 4 Pages
    Better Essays
  • Good Essays

    Science Experimeny

    • 1617 Words
    • 7 Pages

    Cells make hydrogen peroxide as a normal product of metabolism. But it is toxic to the cell if it builds up. Therefore, all cells produce catalase to get ride of hydrogen peroxide. In the following experiment, we will use liver as a source of catalase.…

    • 1617 Words
    • 7 Pages
    Good Essays
  • Good Essays

    The purpose of this lab was to compare the action of a catalyst (enzyme) under different environmental conditions. This was determined by performing a variety of different experiments. The first experiment was performed by adding hydrogen peroxide to sand. Due to the fact that the sand was not soluble in the hydrogen peroxide, no reaction thus no catalyst were present. Manganese dioxide was also added to the hydrogen peroxide creating a moderately fast reaction thus leading to believe that an enzyme was present to lower the activation energy and speed up the reaction.…

    • 446 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Catalase Lab Report

    • 370 Words
    • 2 Pages

    Purpose: To observe the effects of hydrogen peroxide on living tissue and non-living materials, showing the presence or absence of catalase as observed by the presences of bubbles.…

    • 370 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    This experiment will investigate the effect of temperature on the rate of catalase activity by measuring the number of oxygen gas bubbles produced.…

    • 849 Words
    • 4 Pages
    Satisfactory Essays
  • Good Essays

    The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes, temperature, and PH level).…

    • 808 Words
    • 4 Pages
    Good Essays