Collection and Preparation of Materials
The bark of Santol (Sandoricum koetjape (Burm.f.) merr.) were collected at the researchers backyard on Rosales, Province of Pangasinan. The collection was done in the month of August to September 1999.
The bark was collected through the used of a knife. The bark were dried under the sun. And were placed into container and tightly closed ready for expression.
Preliminary Test for the Determination of Plant Constituent It was done to conduct a phytochemical screening of a particular plant and the identification of plant constituent present in its extract.
Preparation of the Plant Extract Place 10g of dried ground plant sample or 20g of fresh sample in an erlenmeyer flask covered with a funnel. To this, add 100ml of 80% ethyl alcohol. Place the flask over a hot water bath and reflux for an hour. If exhaustive extraction is desired, boil the sample for ten minutes only, macerate in an olender and then continue alcohol extraction for an hour. Pour the mixture while hot through a Bunhmer funnel line with filter paper and fitted to a suction flask. Rinse the flask and plant material with fresh portions of 80% ethanol. Include rinsing with the extract. Concentrate the extract to about 15ml and keep in a tightly stopper container. Concentrated extracts should be stored with a trace of toluene or chloroform to prevent fungal growth.
A. Test for Alkaloids
a. Preliminary Alkaloid Test
Take an equivalent of 2ml of plant sample. Evaporate to a syrupy consistency. Add 5ml of 2M HCL to the extract, stir while heating for 5 minutes and then cool. Add about 0.5g powdered NaCl while heating for 2 minutes. Cool and filter the mixture. Add enough fresh 2M HCl to wash the residue and then bring the volume of the filtrate to 5ml. Place 1ml each of the filtrate to 2 test tubes as follows:
1. To 1ml of the filtrate, add a few drops of Mayer’s Reagent.
Result: Light brown solution with