Experiment 4 Lab Review

Topics: Glucose, Carbohydrate, Protein Pages: 2 (637 words) Published: January 29, 2015
Experiment #4 (10/31/14) Lab Review

Objective
To introduce the basic molecules of life and to test for their presence in different substances. Introduction
Macromolecules are in all forms of life.  These organic compounds are carbohydrates, lipids, proteins, and nucleic acids.  These are monomers and they link together into long chains that form polymers.  Different reagents can be used to find the presence of these macromolecules. Proteins are made of carbon, hydrogen, oxygen, nitrogen, and sometimes sulfur.  Proteins are covalently bonded in chains as polymers of amino acids.  These bonds are called peptide bonds.  Amino acids linked together form a polypeptide called a protein.  Some proteins are also enzymes.  The Biuret’s test is only for protein. Carbohydrates contain carbon, hydrogen, and oxygen.  They offer energy and also provide cell support in cells.  There are three classifications for Carbohydrates: monosaccharides, disaccharides, and polysaccharides.  Monosaccharides are the simple sugars.  Two monosaccharides make a disaccharide.  Three or more monosaccharides are a polysaccharide.  Glucose, fructose, and galactose are monosaccharides.  Sucrose, lactose, and maltose are disaccharides.  Starch and glycogen are polysaccharides.  Simple sugars can be found by using Benedict’s test. Starch will react with IKI to give a dark blue color change. Lipids are macromolecules that are usually insoluble in water.  Lipids are made of carbon, hydrogen, and oxygen.  They are referred to as fats or triglycerides.  Oils are liquid at room temperature and are called unsaturated.  Solid fats are saturated.  Lipids are found in cell membranes and are an energy source.  By using the Sudan III test the presence of lipids can be found. Data

Part 1 – Testing for Protein

Description
Observations
1
Egg white / albumin
Purple, 8 drops
2
Pepsin solution
Purple, 8 drops
3
Sugar
No color change
4
Water
No color change
The Biuret reagent contains sodium...
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