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10.0 EXPERIMENT ON DETERMINATION OF DISSOLVED OXYGEN
Sl. No.

Contents
Preamble

10.1

Aim

10.2

Introduction
10.2.1

Environmental Significance

10.3

Principle

10.4

Materials Required
10.4.1

Apparatus Required

10.4.2

Chemicals Required

10.5

Sample Handling and Preservation
10.5.1

10.6

Precautions
Procedure

10.6.1

Preparation of Reagents

10.6.2

Testing of Water Sample

10.7

Calculation
10.7.1

Table

10.7.2

Data Sheet

10.8

Interpretation of Results

10.9

Inference

10.10

Evaluation

10.0 EXPERIMENT ON DETERMINATION OF DISSOLVED OXYGEN
PREAMBLE:
“How to determine dissolved oxygen in Water and Wastewater”.
Test procedure is in accordance to IS: 3025 (Part 38) - Reaffirmed 2003.
In addition to our Indian Standard, we also discuss in brief regarding the procedure stated in
(1) APHA Standard Methods for the Examination of Water and Wastewater - 20th
Edition. Method 4500-O G.
(2) Methods for Chemical Analysis of Water and Wastes, EPA-600/4-79-020,
USEPA, Method 360.1.
10.1 AIM
To determine dissolved oxygen (DO) in the given water sample with the stipulations as per IS: 3025 (Part 38) - Reaffirmed 2003.
10.2 INTRODUCTION
Before performing this experiment, few questions may arise to the learners:
1. What is meant by Dissolved Oxygen (DO)? Is it oxygen in dissolved form?
2. Why we need to determine DO?
3. What are the methods available to determine DO?
4. Is it measured in natural water or wastewater?
5. Whether is it mandatory as per our codal provision to determine DO?
The term Dissolved Oxygen is used to describe the amount of oxygen dissolved in a unit volume of water. Dissolved oxygen (DO) is essential for the maintenance of healthy lakes and rivers. It is a measure of the ability of water to sustain aquatic life. The dissolved oxygen content of water is influenced by the source, raw water temperature, treatment and chemical or biological processes taking place in the distribution system.
The presence of oxygen in water is a good sign. Depletion

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