Int. J. Biosci.
International Journal of Biosciences | IJB |
ISSN: 2220-6655 (Print) 2222-5234 (Online)
Vol. 3, No. 10, p. 8-14, 2013
Evaluation of rice varieties using proteomic approach
Shazia Dilber, Khushi Muhammad*, Inamullah, Muhammad Shahid Nadeem , Fida Muhammad Abbasi, Nazia Akbar, Ikram Muhammad, Habib Ahmad
Department of Genetics, Hazara University Mansehra 21300, Pakistan Key words: Rice, proteomics, SDS-PAGE.
Article published on October 05, 2013
In present study, proteomics approach was used to evaluate three varieties i.e. Bas-385, Indica and KS-282 of rice. Total crude protein was isolated from root, endosperm, embryo and leaf sheath and leaf blade of these varieties and it was separated by SDS-PAGE. The protein bands were scored and used to compare the rice varieties. Seven protein bands in endosperm, six in embryo, seven in roots, six in leaf sheath and seven in leaf blade were recorded conserved proteins during this study. Three protein bands of 25, 30 and 160KDa were detected in the endosperm protein of Bas-385 and JP-5, sharing a common genetics of Basmati and non Basmati. A band of 17KDa was detected in the embryo of Bas-385 while absent in KS-282 and JP-5. Three protein bands i.e. 27, 90 and 120KD were detected in the leaf blade of Bas-385. A band of 45KDa was detected in the leaf blade of JP-5 and absent in KS-282 and Bas-385. A band of 61KDa and three bands of 25, 85 and 175KDa were detected in the embryo and leaf sheath of JP-5 respectively and absent in KS-282 and Bas-385. It is indicating that these proteins could determine the Japonica characters in JP-5. Two bands of 35KDa and 40KD were recorded in endosperm and root of KS-282 respectively while absent in Bas-385 and JP-5. This study explored that there are proteins that are specific for Basmati rice and non Basmati rice or Indica and Japonica rice and could be used to identify rice cultivars.
Author: Khushi Muhammad email@example.comfirstname.lastname@example.org
8 Dilber et al.
Int. J. Biosci.
processes of rice. The genome sequences of rice are
Rice belongs to the family “Poaceae” and genus
completely sequenced the challenge ahead for the
“Oryza Linn” with diploid (2n=24) chromosome. The
plant research community will be to identify the
genus Oryza comprises 25 species distributed
function, regulation, and of each encoded protein.
Gaining an understanding of the biological functions
subtropical region of world. Out of 25 common
of novel genes is a more ambitious goal than
species of Oryza, two species i.e. O. sativa and O.
obtaining just their sequences, however the wealth of
glaberrima are cultivated widely. O. sativa is grown
information on nucleotide sequence that is being
worldwide while O. glaberrima is only confined to
generated through genome projects for outweighs
West Africa (Grist, 1986). It is further sub-divided
what is currently available on the amino acid
into two main types; indica adapted to the tropics and
sequence of known proteins (Lockhart et al., 2000; Li
japonica adapted to temperate regions (Kochko,
et al., 2012)
Genetic engineering provides an efficient and precise
Rice is an important crop in eastern Asia. Rice
breeding tool in which genes of interest (such as Xa21
provides 20 percent of the world’s dietary energy
for bacterial blight resistance, cry for stem borer
supply in different region of the world; in addition
resistance, ORF2 for virus resistance, DREB/TPSP for
drought and salinity tolerance, chi11, RC7, and NPR1
carbohydrates, 8.0g proteins, 0.69g fates and is a
for fungal resistance, psy and crtI for provitamin-A
good source of thiamine, riboflavin and...
References: Anonymous. 2006. data at: http://faostar.fao.rog.
Anonymous. 2008. Global Agriculture Information
Network (GAIN) report
Pakistan Statistical Year book, 2009
Beachy RN. 2003.
Chen LFO, Chen LC. 1989. Inheritance of two
endosperm protein loci in rice (Oryza saliva L.)
Datta SK, Khush GS. 2002.
NE. 2000.Characterization and genomic analysis of
RoCollards TH.2012. Serpins in rice: protein
Virology 81, 1579–1585.
P, Varma H et al. 2002. A Draft Sequence of the
Rice Genome (Oryza sativa L
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