Evaluation of rice varieties using proteomic approach

Topics: Rice, Oryza sativa, SDS-PAGE Pages: 30 (3432 words) Published: October 28, 2013
Int. J. Biosci.

2013
International Journal of Biosciences | IJB |
ISSN: 2220-6655 (Print) 2222-5234 (Online)
http://www.innspub.net
Vol. 3, No. 10, p. 8-14, 2013

RESEARCH PAPER

OPEN ACCESS

Evaluation of rice varieties using proteomic approach
Shazia Dilber, Khushi Muhammad*, Inamullah, Muhammad Shahid Nadeem , Fida Muhammad Abbasi, Nazia Akbar, Ikram Muhammad, Habib Ahmad
Department of Genetics, Hazara University Mansehra 21300, Pakistan Key words: Rice, proteomics, SDS-PAGE.

doi: http://dx.doi.org/10.12692/ijb/3.10.8-14

Article published on October 05, 2013

Abstract
In present study, proteomics approach was used to evaluate three varieties i.e. Bas-385, Indica and KS-282 of rice. Total crude protein was isolated from root, endosperm, embryo and leaf sheath and leaf blade of these varieties and it was separated by SDS-PAGE. The protein bands were scored and used to compare the rice varieties. Seven protein bands in endosperm, six in embryo, seven in roots, six in leaf sheath and seven in leaf blade were recorded conserved proteins during this study. Three protein bands of 25, 30 and 160KDa were detected in the endosperm protein of Bas-385 and JP-5, sharing a common genetics of Basmati and non Basmati. A band of 17KDa was detected in the embryo of Bas-385 while absent in KS-282 and JP-5. Three protein bands i.e. 27, 90 and 120KD were detected in the leaf blade of Bas-385. A band of 45KDa was detected in the leaf blade of JP-5 and absent in KS-282 and Bas-385. A band of 61KDa and three bands of 25, 85 and 175KDa were detected in the embryo and leaf sheath of JP-5 respectively and absent in KS-282 and Bas-385. It is indicating that these proteins could determine the Japonica characters in JP-5. Two bands of 35KDa and 40KD were recorded in endosperm and root of KS-282 respectively while absent in Bas-385 and JP-5. This study explored that there are proteins that are specific for Basmati rice and non Basmati rice or Indica and Japonica rice and could be used to identify rice cultivars.

* Corresponding

Author: Khushi Muhammad  khushisbs@yahoo.com/inamphd@gmail.com

8 Dilber et al.

Int. J. Biosci.

2013

Introduction

processes of rice. The genome sequences of rice are

Rice belongs to the family “Poaceae” and genus

completely sequenced the challenge ahead for the

“Oryza Linn” with diploid (2n=24) chromosome. The

plant research community will be to identify the

genus Oryza comprises 25 species distributed

function, regulation, and of each encoded protein.

throughout

and

Gaining an understanding of the biological functions

subtropical region of world. Out of 25 common

of novel genes is a more ambitious goal than

species of Oryza, two species i.e. O. sativa and O.

obtaining just their sequences, however the wealth of

glaberrima are cultivated widely. O. sativa is grown

information on nucleotide sequence that is being

worldwide while O. glaberrima is only confined to

generated through genome projects for outweighs

West Africa (Grist, 1986). It is further sub-divided

what is currently available on the amino acid

into two main types; indica adapted to the tropics and

sequence of known proteins (Lockhart et al., 2000; Li

japonica adapted to temperate regions (Kochko,

et al., 2012)

the

world

including

tropical

1987).
Genetic engineering provides an efficient and precise
Rice is an important crop in eastern Asia. Rice

breeding tool in which genes of interest (such as Xa21

provides 20 percent of the world’s dietary energy

for bacterial blight resistance, cry for stem borer

supply in different region of the world; in addition

resistance, ORF2 for virus resistance, DREB/TPSP for

100g

79g

drought and salinity tolerance, chi11, RC7, and NPR1

carbohydrates, 8.0g proteins, 0.69g fates and is a

for fungal resistance, psy and crtI for provitamin-A

good source of thiamine, riboflavin and...


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