Eukaryotic Cells Chapter 7 Summary

Chapter 7 * Why do we want to make Eukaryotic cell proteins? * Because when eukaryotic proteins are expressed in prokaryotic cells problems like been unstable, no biological activity and prokaryotic contaminants ( pyrogens) can occur. * Also to make the protein as natural as possible * The same expression vectors as we need for pro * We don’t need shangdolron coz that only for pro
Transformation: in Eukaryotic cells: in humans means that difference in growth characters of cells, for example, the cells get transformed to cancer cells
Transfection: uptake of foreing DNA into eukaryotic cells resulting in inherited change, the same thing as we did in E.coli transformation
Why would we want to use yeast as an
* If it does not have introns then it wont be expressed as much as the onces that have introns * The agents are going to kill the Euk by different actions * A marker Gene prevents the agents from doing the actions that would have lead to Euk death
What do we need in our mRNA in order to have a successful translation * There is no shangodorno in Euk so we just have AUG that starts the translation. There is something that tells the cell its AUG and we find it in the kozak