Enzyme Temperature On Catalase

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The Effect of Increasing Temperatures on the Enzyme Catalase Enzymes are proteins that help speed up chemical reactions inside your body and without enzymes, chemicals reactions in cells would be incredibly slow to a point where no activity at all takes place (Brawo press Inc, 2017). Enzymes speed up the chemical reactions by lowering the activations energy and they do this by binding substrates together in the correct orientation to react (Ernest Z, 2014). They are vital for life and serve a range of important functions in the body such as aiding in digestion and metabolism.

Substrates are the molecules that an enzyme works with and they bind to a region on the enzyme called the active site. Some enzymes help break down molecules in
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It is mostly found in organisms that live in the presence of oxygen and prevents the growth of and protects cellular organelles and tissue damage by peroxide. Catalase is normally found in the liver and in our case, it is found in the lamb liver (EEB, 2015). The equation for this reaction is 2H2O2 → 2H2O + O2, where hydrogen peroxide is broken down into oxygen and water molecules (McDowall, 2004). Temperature has an effect of the structure of the enzyme and the hydrogen bonds. The optimal temperature for catalase is 37˚C. As temperature increases towards the optimal temperature, the hydrogen bonds loosen and this makes it easier for catalase to act on hydrogen peroxide. When the temperature increases above the optimal temperature, the enzyme will denature causing the enzyme to denature and the weak hydrogen bonds are broken (Smith, …show more content…
This will be done by adding detergent to the reaction so there is a clear indication of the rate of reaction.

Therefore, the aim of this experiment is to investigate the effect of increasing temperatures on the activity of the enzyme catalase.

It is hypothesised that when the temperature of the water increases more froth will be observed because the rate of reaction fastens due to the enzymes absorbing more energy. When the temperature exceeds the optimal temperature, the enzymes with start to denature and will slow down the rate of reaction, therefore less froth will be

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