Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A, B, C, and D. In activity A, the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction, to study the characteristics of an enzyme mediated reaction, and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H2O2 present in a solution. The amount will be the baseline for activities C and D. The purpose of activity C is to determine the rate at which H2O2 spontaneously decomposes when exposed to room temperatures and ambient light for 24 hours. The purpose of activity D is to determine the rate at which catalase decomposes H2O2. After adding H2SO4 for different time lashes, etc., the resulting data will be graphed at which the catalase decomposed by catalase.…
|Hot Water |Hot water splashing or spilling on |Safety glasses and aprons were worn |…
Explain in detail the procedure that you followed (including amount of substrate, enzyme etc, and the whole procedure including incubation times) (3 Points)…
The optimum pH for the enzyme acid phosphatase was predicted to be within acidic regions and the results obtained showed that the optimum pH was about 5.5 see fig.10. It had the highest absorbance value, meaning it had the most PNP in the tube in the given time and thus the fastest rate of reaction. A change in pH changes the shape of the active site of the enzyme. The bonds within the active site of the enzymes are polar, this means that they are extremely sensitive to ions. The decrease in pH increases the concentration of H+ ions in the solutions, these interact with the polar bonds in the enzymes structure to form individual bonds. This disrupts the shape of the active site and thus the substrate PNPP is no longer complementary to the enzyme’s active site. So no Enzyme substrate complexes can be formed and the rate of reaction drops. The same thing happens when there are extra OH- ions in the mixture. The pH in our cells must be extremely specific and buffered in order to prevent changes in pH and the denaturing of these enzymes. The data collected during these experiments are very similar to those published and studied, meaning the results collected are valid, and thus the experiment…
The results of our experiment showed the solutions in both tube 1 and tube 2 increasing in absorbency in the first eight minutes but then tube 1 continued to increase while tube 2 began to balance out. Tube 3, our blank, managed to stay at 0nm the entire twenty minutes. From this data, we can conclude that our hypothesis was supported that EDTA had a greater change in absorption over PTU.…
Prediction: As the temperature increases the rate of enzyme activity will also increase, thus increasing the rate of reaction. However, if the temperature is too high the enzyme will denature.…
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Problem: How can we demonstrate how enzymes work? What happens if we alter the environment of an enzyme?…
Enzymes are proteins that are involved in all the chemical processes in living things. As they are made of proteins they are affected by pH and temperature. Enzymes are catalysts; they speed up chemical reactions without being changed themselves. Digestive enzymes speed up the breakdown of large food molecules into smaller ones so that the blood can absorb them. Enzymes turn a large starch molecule into thousands of tiny glucose molecules. Enzymes end in 'ase'. There are thousands of enzymes in our body but each enzyme is only specialised to do one thing, for example carbohydraise enzymes digest carbohydrates, protease enzymes digest protein.…
Title: The Effect of Adjusted Concentration of Hydrogen Peroxide on the change in reaction rate of liver catalase.…
Abstract: The Enzyme Lab results where when the liver was frozen, its reaction was fast, and when it was hot, it was slow, and the liver that was at room temperature reacted slowly to medium.…
experiment is to determine what changes in pH, temperature, and enzyme concentration have on the rate…
Enzyme B produced the most maltose at a high temperature (100 ºC) and an acidic pH.…
Enzymes are a protein serving as a catalyst, a chemical agent that changes the rate of the reaction without being consumed by the reaction. Enzymes are proteins made up of long chains of amino acids. These form complex shapes. The enzymes are individuals, like the different players on a ball team, they have different specific structures and jobs. As one ball player may be very tall and one short, the specific different shape of the active site on an enzyme is unique and prepares it to mix with a certain substrate. Without enzymes, the process of metabolism would be hopelessly slow. The reactant an enzyme acts on is referred to the enzyme 's substrate. The enzyme will combine with or to its substrate. While the two are joined, the substrate is converted to its product by catalytic action of the enzyme. There is an active site of the enzyme molecule which is a restricted region that actually attaches to the substrate. Usually the active site is formed by only a few of the enzyme 's amino acids, the rest is just the framework that reinforces the active site. In an enzymatic reaction, the substrate enters the active site then is held in place by weak bonds. Now the enzyme does its work and first changes shape so it can hold onto the substrate. Next the substrate is changed to its product, the product is released and the enzymes active site is ready and waiting for another molecule of substrate.…
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