Enzyme Project

Better Essays
BABS1201 Group Enzymes Project
Group Protocol Including Equipment and Reagents List

Lab Day: Wednesday
Lab Time: 10am – 1pm
Are you in Lab G20 (furthest from BSB Student Office) or Lab G21 (closest to BSB Student Office)?:
Demonstrator Name: Daniel Winters
Names of Group Members: Johnny Nguyen, Therese Pham, Linda Tang
Name of Enzyme You are Investigating: Amylase
Brief Background:
Amylase is a digestive enzyme, produced mainly by the salivary glands and the pancreas, to break down starch in food into smaller carbohydrate molecules and disaccharides such as maltose. It can be found in humans and some other mammals. Some plants and bacteria may also produce amylase. After being broken down into smaller carbohydrate molecules, it can be converted into a monosaccharide such as glucose, which fuels processes for organism function. There are two variations of this molecule but the human body has alpha amylase. Food that contains large amounts of starch will have a slightly sweet taste when chewed because of this breaking down of starch into sugar by amylase. Alpha Amylase 's official name is 1,4-a-D-Glucan glucanohydrolase; EC 3.2.1.1. It breaks down starch into maltose via hydrolysis. Amylase is folded into a tertiary shape and has an area called the “active site” where the substrate, carbohydrate, is broken down. Its structure consists of a single chain of amino acids, which forms three distinct regions called domains, where each domain has a specific biological function assisting in the breakdown of carbohydrates. Several amylases require the presence of a cofactor to function. A cofactor is a chemical species necessary for the enzyme to function but does not necessarily undergo chemical reaction. Amylase requires calcium and chloride ions for activity in animals and plants. The amylase from the bacteria Bacillus subtilis contains zinc ions.
Hypotheses:
Substrate Concentration
In the more concentrated starch solution, there will be an increase in



References: http://www.eng.umd.edu/~nsw/ench485/lab5.htm http://www.123helpme.com/view.asp?id=121479

You May Also Find These Documents Helpful

  • Powerful Essays

    What is Enzyme? Enzymes are protein that acts as speed up reactions and break down molecules in our body. However, different enzymes only work on certain types of molecules. Enzymes can accelerate the reactions by more than one million times.(3) In our human body, there are a total about forty thousand types of enzymes and each catalyzes different kind of molecule.(3) The molecules that enzymes help to accelerate is called substrates, and when enzyme is combined together with the substrate, it…

    • 1115 Words
    • 5 Pages
    Powerful Essays
  • Good Essays

    ENZYMES

    • 1134 Words
    • 5 Pages

    it's lowest to do so. However, in cold solutions the starch will take longer as it will in temperatures beyond 40 degrees. Once it reaches this point, the break down will either take a very long period of time or have no reaction at all as enzymes are denatured at a certain point. Materials: · 4 x test tubes · 5mL Diastase · 5mL Water · 10mL 2% Starch Suspension. · Pipette · 2 x Spotting tiles · Large Beaker filled with water of assigned temperature · Thermometer…

    • 1134 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Enzymes

    • 535 Words
    • 3 Pages

    Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free…

    • 535 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Enzymes

    • 1015 Words
    • 5 Pages

    amylase and starch. Introduction The enzyme amylase is found in the human body, it catalyses the hydrolosis of internal glycosidic bonds in polysaccharides, the breakdown of starch into sugars. Amylase is present in human saliva, where it initiates the chemical process of digestion. Enzymes work best at an optimum pH of 7 which is the bodies normal pH. The pH affects the charge of the amino acid at the active site. PH changes affect the structure of an enzyme molecule and therefore affect its ability…

    • 1015 Words
    • 5 Pages
    Good Essays
  • Satisfactory Essays

    Enzymes

    • 390 Words
    • 2 Pages

    Enzymes (pron.: /ˈɛnzaɪmz/) are large biological molecules responsible for the thousands of chemical interconversions that sustain life.[1][2] They are highly selective catalysts, greatly accelerating both the rate and specificity of metabolic reactions, from the digestion of food to the synthesis of DNA. Most enzymes are proteins, although some catalytic RNA molecules have been identified. Enzymes adopt a specific three-dimensional structure, and may employ organic (e.g. biotin) and inorganic (e…

    • 390 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Enzyme

    • 508 Words
    • 3 Pages

    There are approximately 40,000 enzymes living in one human cell, each responsible for a chemical reaction. Enzymes are complex 3D protein molecules created by amino acids, forming a unique sequence that produces hydrogen bonds, eventually formulating an enzyme within plants and animals (Boyle & Senior, 2002). Working alongside other molecules, they uphold a stable reaction system. The function of an enzyme is to aid and increase chemical reactions and organise metabolism, while maintaining homeostasis…

    • 508 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Enzyme

    • 1845 Words
    • 7 Pages

    milk Introduction Enzymes are globular protein, responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon, hydrogen, oxygen and nitrogen (Gunsch, 2012). The role of enzyme is to act as catalysts, substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done by lowering the activation energy required to start a reaction. Enzymes are specific in…

    • 1845 Words
    • 7 Pages
    Better Essays
  • Satisfactory Essays

    Enzymes

    • 572 Words
    • 5 Pages

    Biology “Enzyme Activities” Introduction: Enzymes have extremely interesting properties that make them little chemical-reaction machines. The purpose of an enzyme in a cell is to allow the cell to carry out chemical reactions very quickly. These reactions allow the cell to build things or take things apart as needed. This is how a cell grows and reproduces. At the most basic level, a cell is really a little bag full of chemical reactions that are made possible by enzymes (Brain). Laboratory…

    • 572 Words
    • 5 Pages
    Satisfactory Essays
  • Powerful Essays

    Enzymes

    • 3005 Words
    • 13 Pages

    reactions Keywords: Alkaline phosphatase; kinetics; Enzyme-cofactor interaction; synergism * corresponding author. Email: femijohn@gmail.com 43 INTRODUCTION The roles of metal ions in metalloenzymes include direct participation in catalysis, stabilization of protein structure and regulation of enzymatic activity. Membrane alkaline phosphatase (ALP) is a metal-containing enzyme that serves as a good model for the study of metal ion interactions in enzyme catalysis. Native E. coli ALP contains three…

    • 3005 Words
    • 13 Pages
    Powerful Essays
  • Powerful Essays

    Enzymes

    • 2259 Words
    • 10 Pages

    __________________________________ AP Biology LAB 2. ENZYME CATALYSIS ESSAY 2000 The effects of pH and temperature were studied for an enzyme-catalyzed reaction. The following results were obtained. a. How do (1) temperature and (2) pH affect the activity of this enzyme? In your answer, include a discussion of the relationship between the structure and the function of this enzyme, as well as a discussion of how structure and function of enzymes are affected by temperature and pH. b. Describe…

    • 2259 Words
    • 10 Pages
    Powerful Essays