Enzyme Lab Report Essay

After conducting the experiment and gathered the required data, we came to conclusion that certain environmental factors affect the enzyme activity rate. For the first experiment, where we tested the increase in concentration of enzyme with the substrate, we found that higher concentration of enzyme increases the rate of reaction of the enzyme. This is because more enzyme molecules are present, which allow more substrate molecules to get into the active sites of the enzyme (Sattler W& Esterbauer H). When calculating the absorbance of different enzyme concentration, it was noticeable that the absorbance decreased as time increased; however, at a certain time it decreased and then increased. This might have occurred because we have done something wrong either we added more solution or the cuvette had finger print. However, our results confirm our hypothesis that higher concentration of enzyme cause an increase in the rate of reaction at a certain point. The graph of “Enzyme's Concentration Effect on a Substrate Concentration “illustrates the slopes of the lines of different enzyme concentration. It is apparent that the slope of the % amylose of undiluted enzyme was fluctuating, whereas, the % amylose of 1:9 undiluted enzyme
The reason behind that is because changes in pH level change the shape of the enzyme which ultimately affect the effectiveness of the rate of reaction, either increase it or decrease it. Lower or higher pH level than pH 6 will lower the rate of reaction. Looking at the graph of “Enzyme Effected by pH Levels” we can see the slope of pH6 was increasing and then it gradually decreased. One of the limitation we encountered was the inaccuracy of the data collected, which we solved by redoing the