Enzyme Lab Report
Danny Fish
10/9/11
Chemical Testing To identify An Unknown
The hypothesis tested was that depending on the solution presented, which would test positive for one of the following, proteins, carbohydrates, or lipids through use of chemical testing. (Sudan IV, Benedicts’ Solution, Iodine, Biuret’s) . In order to gain more information for the hypothesis, one must know how to test for said macromolecule. Each of the above stated molecules has their own individual solution that will in turn identify which molecule is present. The corresponding test are as followed; Sudan IV is used for testing for lipids, if in fact a lipid is present, the reaction would produce a red/orange color as opposed to no change at all (negative reaction: Stays pink color). This solution of Sudan IV is soluble in lipids, but not in water. Benedict’s solution is the test used for reducing sugars or glucose testing. The positive reaction for this test should show a red/brick color after being placed in boiling water for three minutes, but if a negative reaction occurs we will get blue color or no change at all. Another test that we were introduced to was the iodine test, which is used to detect starch. A positive reaction would result in a blue/black color, where as a negative reaction would be an amber color. Then in order to find the remaining agents (peptide bonds) we used Biuret’s solution, a violet color is produced when there is a positive reaction. Where as a light blue color is produced in the negative reaction. The overall goal of this experiment is to find three of the four macromolecules (proteins, lipids, carbohydrates) that you can find in everyday living organism. These macromolecules are the chain like proteins, carbohydrates, and nucleic acid. These macromolecules are made of smaller units that are called monomers that make up polymers of covalently boding the identical and/ or similar monomer building blocks. These monomers are repeating units serving as building blocks
10/9/11
Chemical Testing To identify An Unknown
The hypothesis tested was that depending on the solution presented, which would test positive for one of the following, proteins, carbohydrates, or lipids through use of chemical testing. (Sudan IV, Benedicts’ Solution, Iodine, Biuret’s) . In order to gain more information for the hypothesis, one must know how to test for said macromolecule. Each of the above stated molecules has their own individual solution that will in turn identify which molecule is present. The corresponding test are as followed; Sudan IV is used for testing for lipids, if in fact a lipid is present, the reaction would produce a red/orange color as opposed to no change at all (negative reaction: Stays pink color). This solution of Sudan IV is soluble in lipids, but not in water. Benedict’s solution is the test used for reducing sugars or glucose testing. The positive reaction for this test should show a red/brick color after being placed in boiling water for three minutes, but if a negative reaction occurs we will get blue color or no change at all. Another test that we were introduced to was the iodine test, which is used to detect starch. A positive reaction would result in a blue/black color, where as a negative reaction would be an amber color. Then in order to find the remaining agents (peptide bonds) we used Biuret’s solution, a violet color is produced when there is a positive reaction. Where as a light blue color is produced in the negative reaction. The overall goal of this experiment is to find three of the four macromolecules (proteins, lipids, carbohydrates) that you can find in everyday living organism. These macromolecules are the chain like proteins, carbohydrates, and nucleic acid. These macromolecules are made of smaller units that are called monomers that make up polymers of covalently boding the identical and/ or similar monomer building blocks. These monomers are repeating units serving as building blocks
Cited: 1. Lisa Urry, Jane Reece,Michael Cain, Steve A Wasserman,Robert Jackson,Peter Minorsky, Neil Campbell. Campbell Biology Ninth Editoin. San Francisco. Pearson Education Inc.2011 2. Stretched exponential dynamics in lipid bilayer simulations. Brandt, Erik G. Journal of Chemical Physics; 9/21/2010, Vol. 133 Issue 11, p115101, 12p, 1 Diagram, 3 Charts, 9 Graphs 3. The behavior of reorientational correlation functions of water at the water-lipid bilayer interface. Bhide, Shreyas Y.Berkowitz, Max L. Journal of Chemical Physics; 9/7/2006, Vol. 125 Issue 9, p094713, 7p, 5 Charts, 5 Graphs 3. Biology Laboratory Manual. Darrell S. Vodopich, Randy Moore. McGraw-Hill. New York, NY 10020. 2011