enzyme lab

Good Essays
Topics: PH
Lab 6.C
Hypothesis: If enzyme activity is affected by the pH of a solution, then the enzymes will experience the greatest activity at a pH of 6.
Variables:
Independent Variables
Dependent Variable
Controls
Four different pH values (10, 7, 6, and 3)
Change in color of the solution
The amount of potato extract, pH solution, and catechol used (1 cm +/- .1cm)
Size of the test tubes
Amount of time allowed for the catechol to sit with the potato extract and pH solution (20 minutes with 5 minute intervals for observation)

Materials:
4 test tubes
Test tube rack
Wax pencil
Small ruler
Buffer solutions of pH 10, 7, 6, and 3
Potato extract
Catechol
Procedure:
1. Using the small ruler and the wax pencil, three markings are made on 3 test tubes. These markings should be at 1, 2, and 3 cm from the bottom of the tube. These tubes are then numbered by their pH values: 10, 7, 6, and 3.
2. One potato is chopped into small pieces and placed into a blender.
3. 125mL of distilled water is added to the potato.
4. This solution is blended on high just long enough to break open the cells, approximately 5 seconds.
5. This is then strained through 3 layers of cheese cloth into a beaker and kept cold in order to help prevent oxidation of the enzymes.
6. Each tube is filled up to the 1 cm mark with the solution matching the pH written on it.
7. Each test tube is then filled up to the 2 cm mark with potato extract and mixed.
8. Next, each tube is filled to the 3 cm mark with catechol and mixed well.
9. Any color changes are observed and documented using a plus and minus scale at 5 minute intervals for a total of 20 minutes.
Raw Data:
Change in Color of Potato Extract with Different pH Values over Time Based on Color Intensity Scale Initial Observations
5 Minutes
10 Minutes
15 Minutes
20 Minutes
Tube 10
Transparent light red
-
-
-
-
Tube 7
Gold
++
+++
+++
+++
Tube 6
Light gold
+
++
+++
++++
Tube 3
Light yellow
+

You May Also Find These Documents Helpful

  • Good Essays

    Enzyme Lab

    • 938 Words
    • 4 Pages

    Enzyme Activity Lab Hypothesis: 1] Are enzymes reusable? Yes, enzymes are reusable. 2] Which of the following do you think contain catalase? [Manganese dioxide, chicken meat, apple, carrot, potato, or chicken liver] I think that the manganese dioxide, apple, and carrot contain the catalase. 3] Would grinding the materials affect enzyme activity? I think that grinding the materials will increase enzyme activity. 4] Would temperature affect enzyme activity? I think that…

    • 938 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    Enzyme Lab

    • 1094 Words
    • 5 Pages

    MISEP Chemistry 512 – Jacobs Enzyme Catalyst Lab - Formal Report – August 8, 2007 ABSTRACT This investigation examined what would happen to the rate of an enzyme-catalyzed reaction if the concentration of substrate changed. We hypothesized that if the concentration increased, then the reaction rate would also increase. To test our question, we varied a combination of substrate and buffer, totaling 6mL, with a constant amount of 2 drops of catalyst. The enzyme catalyst, peroxidase, increased…

    • 1094 Words
    • 5 Pages
    Powerful Essays
  • Satisfactory Essays

    Enzyme Lab

    • 338 Words
    • 10 Pages

    Effect of the Changes in the Environment to the Functionality of the Enzymes Introduction a. Background In our everyday lives, enzymes are used in our bodies, and in nature around us, to speed up the chemical reactions happening constantly, which happens by lowering the amount of activation energy needed to start various reactions. The way this works is by attaching the particular substrate to the active site of the enzyme, where it will start to aid the chemical reaction. Then, the allosteric…

    • 338 Words
    • 10 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Enzyme Lab

    • 353 Words
    • 2 Pages

    Enzyme Lab Marcus James. December 20, 2013 HL Biology 3 Period 1 Purpose and Background The purpose of this lab is to explain how enzymes act as catalysts for biological reactions in different temperatures. This lab relates to enzymes, proteins, and substrates; that we learned in class. The union of the enzyme and the substrate is called the enzyme-substrate complex. The make-up of an enzyme is proteins and made up of chains and amino acids. Enzymes…

    • 353 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Enzyme Lab

    • 842 Words
    • 4 Pages

    Lab Report: Enzyme Lab Your Name: Samantha Butcher Purpose of this Lab What is the goal of this lab? What question is it trying to answer, or what problem is it trying to explain? It is going to explain how a liver's enzymes break down substances that could be harmful.| Hypothesis After reading the lab instructions - but before starting the lab - record your best “educated guess” about what will happen in the experiment. Give your reasons and outline any assumptions that lead…

    • 842 Words
    • 4 Pages
    Good Essays
  • Good Essays

    enzyme lab

    • 344 Words
    • 2 Pages

    of the lab is to see the effects of enzyme concentration on the reaction rate. Part 2: Effect of Substrate Concentration Purpose: The purpose of this part of the lab was to find the effect of substrate concentration on enzyme activity. Part 3: Effect of Temprature Purpose: The purpose of this part of the lab was to determine the effect of temperature on the rate of enzyme activity…

    • 344 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Enzyme Lab

    • 725 Words
    • 3 Pages

    Enzymes play a vital role in helping our body function. They act as biological catalysts and help speed up reactions that would otherwise take long periods of time to naturally occur. Enzymes help lower the activation energy required for the reactants to reach the transitional state from which then they can form products. However, enzymes do not change the free energy of the reaction. Enzyme’s ability to catalyze reactions comes from the shape of the active site on the enzyme. Enzymes are hyper-specific…

    • 725 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Enzyme Lab

    • 716 Words
    • 3 Pages

    Sample Lab Report: Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include:  background information about concepts involved in the lab,  statement of purpose of the lab  identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in…

    • 716 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Enzyme Lab

    • 1965 Words
    • 57 Pages

    20th, 2012 Enzyme Lab What is an enzyme? Enzymes are specialized protein molecules simplifying most of the body's metabolic processes such as, supplying energy, digesting foods, purifying your blood, executing the body of waste products etc. Enzymes act as catalyst by speeding up the reactions that happen in our bodies and decreasing the amount of activation energy needed to break a complex down. A reactant is any given enzymatic reaction is called a substrate for that specific enzyme. The place…

    • 1965 Words
    • 57 Pages
    Better Essays
  • Satisfactory Essays

    Enzyme Lab

    • 431 Words
    • 2 Pages

    Enzyme Lab: Peter Kuetzing – 10/4/2012 – F Block 1. How does changing the concentration of enzyme affect the rate of decomposition of H2O2? When more enzymes is added the rate of reaction speeds up. In this case, H2O2 will produce more O2, in return the kpa/min will go up. 2. What do you think will happen to the rate of reaction if the concentration of enzyme is increased to five drops? Predict what the rate would be for 5 drops. I think that the rate of reaction will slightly increase from…

    • 431 Words
    • 2 Pages
    Satisfactory Essays