Enzyme Controlled Reaction
Enzyme-Controlled Reaction
Procedure:
Click the TV/VCR. Then click the Play button on the video controller. Watch an animation about enzyme action.
Click More Information to read about enzymes and substrates.
To conduct the experiment:
Adjust the pH level of the test tube by click the up and down arrows
Add substrate to each of the test tubes that already contain an enzyme solution
Click and drag a piece of weighing paper with the powdered substrate to a test tube.
Click the computer monitor to see the digital counter table that displays the number of product molecules formed during the first minute in each of the five test tubes. This is the initial reaction rate of this enzyme catalyzed reaction.
Click the table button to record the data in the table
Click the rest button.
Repeat the experiment using different amounts of substrate at a constant pH value.
Or use one substrate at five different pH levels.
Record results in the Table.
When all the data have been collected and recorded, click the Graph button to see a graph of the results.
Recorded Data: Enzyme-Catalyzed Reactions
Amount of Substrate pH3 pH5 pH7 pH9 pH11 0.5 g
19/min
39/min
72/min
45/min
24/min
1.0 g
39/min
81/min
145/min
91/min
49/min
2.0 g
82/min
168/min
300/min
189/min
103/min
4.0 g
96/min
198/min
350/min
223/min
121/min
8.0 g
96/min
198/min
350/min
223/min
121/min
Figure 1 Enzyme-Catalyzed Reaction
Journal Questions:
1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases?
The reaction goes faster initially. The relationship is linear. As the substrate concentration increase the initial reaction goes faster.
2) What is the maximum initial reaction rate for this enzyme at pH 7?
The maximum initial reaction rate at pH7 is 350/min.
3) Explain why the maximum initial reaction rate cannot be reached at low substrate
Procedure:
Click the TV/VCR. Then click the Play button on the video controller. Watch an animation about enzyme action.
Click More Information to read about enzymes and substrates.
To conduct the experiment:
Adjust the pH level of the test tube by click the up and down arrows
Add substrate to each of the test tubes that already contain an enzyme solution
Click and drag a piece of weighing paper with the powdered substrate to a test tube.
Click the computer monitor to see the digital counter table that displays the number of product molecules formed during the first minute in each of the five test tubes. This is the initial reaction rate of this enzyme catalyzed reaction.
Click the table button to record the data in the table
Click the rest button.
Repeat the experiment using different amounts of substrate at a constant pH value.
Or use one substrate at five different pH levels.
Record results in the Table.
When all the data have been collected and recorded, click the Graph button to see a graph of the results.
Recorded Data: Enzyme-Catalyzed Reactions
Amount of Substrate pH3 pH5 pH7 pH9 pH11 0.5 g
19/min
39/min
72/min
45/min
24/min
1.0 g
39/min
81/min
145/min
91/min
49/min
2.0 g
82/min
168/min
300/min
189/min
103/min
4.0 g
96/min
198/min
350/min
223/min
121/min
8.0 g
96/min
198/min
350/min
223/min
121/min
Figure 1 Enzyme-Catalyzed Reaction
Journal Questions:
1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases?
The reaction goes faster initially. The relationship is linear. As the substrate concentration increase the initial reaction goes faster.
2) What is the maximum initial reaction rate for this enzyme at pH 7?
The maximum initial reaction rate at pH7 is 350/min.
3) Explain why the maximum initial reaction rate cannot be reached at low substrate