Enzyme Concentration Lab Report
Chirag Patel
Biology 157
Determining the Effect of Varying Substrate Concentration
Question: How does concentration of potatoes affect the amount of oxygen produced with hydrogen peroxide and potatoes (enzymes)?
Introduction The enzyme used for this experiment is Catalase. Catalase is inside mostly any living organism which uses oxygen. Its job is to break down hydrogen peroxide, into oxygen and water. (Formula) 2H2O2 ---> 2H2O + O2 (lab manual). There are limiting factors which if altered, can alter the procedure of the reaction, such as temperature, pH, and the concentration of either the enzyme or the substrates. Enzymes are specialized class of protein that acts as catalysts for the chemical processes within our cell which …show more content…
Since oxygen is one of the products, it is possible to collect it over time and calculate the rate at which the reaction is progressing. As O2 is evolved in the gas bottle, it passes through the tube into the graduated cylinder displacing the water. The number of mls of oxygen in the graduated cylinder can be recorded at regular intervals and then plotted on graph paper to show the rate of the reaction. (Lab manual) Series of trials the concentration of the catalase will be kept constant.
Method
For this series of trials, the concentration of the catalase will be kept constant. You are to choose a catalase concentration which gave a controlled reaction in the previous experiment and use it for this series of trials. Remember that 3% hydrogen peroxide is three times as concentration 1% and should give a more robust response. Beaker i-4 will contain the enzyme concentration that you choose. The temperature will be the same as in the previous experiment. The variable will be the concentration of hydrogen peroxide.(lab
Biology 157
Determining the Effect of Varying Substrate Concentration
Question: How does concentration of potatoes affect the amount of oxygen produced with hydrogen peroxide and potatoes (enzymes)?
Introduction The enzyme used for this experiment is Catalase. Catalase is inside mostly any living organism which uses oxygen. Its job is to break down hydrogen peroxide, into oxygen and water. (Formula) 2H2O2 ---> 2H2O + O2 (lab manual). There are limiting factors which if altered, can alter the procedure of the reaction, such as temperature, pH, and the concentration of either the enzyme or the substrates. Enzymes are specialized class of protein that acts as catalysts for the chemical processes within our cell which …show more content…
Since oxygen is one of the products, it is possible to collect it over time and calculate the rate at which the reaction is progressing. As O2 is evolved in the gas bottle, it passes through the tube into the graduated cylinder displacing the water. The number of mls of oxygen in the graduated cylinder can be recorded at regular intervals and then plotted on graph paper to show the rate of the reaction. (Lab manual) Series of trials the concentration of the catalase will be kept constant.
Method
For this series of trials, the concentration of the catalase will be kept constant. You are to choose a catalase concentration which gave a controlled reaction in the previous experiment and use it for this series of trials. Remember that 3% hydrogen peroxide is three times as concentration 1% and should give a more robust response. Beaker i-4 will contain the enzyme concentration that you choose. The temperature will be the same as in the previous experiment. The variable will be the concentration of hydrogen peroxide.(lab