Enzyme Activity: Laboratory Report

LABORATORY REPORT
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Activity:
Name:
Instructor:
Date:

Enzyme Activity cheryl yelton
November 30, 2014

Predictions
1. Sucrase will have the greatest activity at pH 6
2. Sucrase will have the greatest activity at
40 °C (104 °F)
3. Sucrase activity increases with increasing sucrose concentration.

Materials and Methods
Effect of pH on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time

Effect of Temperature on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) present; sucrase + sucrose incubation time

Effect of Substrate Concentration on Enzyme Activity.
1. Dependent Variable. amount of product (glucose and fructose) produced
2. Independent Variable. amount of substrate (sucrose) present
3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time
4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. undefined 5. Explain why denatured sucrase was used as a control. undefined 6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration. undefined Results
Table 1: Effect of pH on Sucrase Activity
1
2
3
average

ph 3
0.1558
0.1901
0.1653
0.17

ph 4
0.325
0.302
0.311
0.313

Optical Density ph 6
0.954
0.939
0.955
0.949

ph 5
0.644
0.66
0.647
0.65

ph 7
0.775
0.82
0.786
0.794

ph 8
0.409
0.39
0.397
0.399

ph 9
0.194
0.175
0.19
0.186

Effect of pH on Sucrase Activity

1. State the optimum pH for sucrase activity and describe how sucrase activity changes at more acidic and more alkaline pH