Enzyme Activity
LABORATORY REPORT
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Activity: Name: Instructor: Date:
Enzyme Activity Pam Campbell Id 0002337 Dr. Murphy Nmezi August 9, 2011
Predictions
1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached.
Materials and Methods
Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time
Effect of Temperature on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) present; sucrase + sucrose incubation time
Effect of Substrate Concentration on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. amount of substrate (sucrose) present 3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time 1. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. Glucose is measured as an indicator of sucrase activity, as glucose is a producut produce by sucrase activity. 2. Explain why denatured sucrase was used as a control. Denatured sucrase was used as a control to determine if a true effect was made on the active sucrase by pH, temperature, and sucrose, or if affect would be seen in both the denatured and active sucrase.
3. Alkaline DNS denatures sucrase. Explain why it is important to denature the enzyme. It is important to determine if pH, temperature, and sucrose do have a true effect on active sucrase.
Results Table 1: Effect of pH on Sucrase Activity
1 2 3 average ph 3 0.171 0.159 0.166 0.165
(Click on the Save a Copy button on the panel above to save your report)
Activity: Name: Instructor: Date:
Enzyme Activity Pam Campbell Id 0002337 Dr. Murphy Nmezi August 9, 2011
Predictions
1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached.
Materials and Methods
Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time
Effect of Temperature on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) present; sucrase + sucrose incubation time
Effect of Substrate Concentration on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. amount of substrate (sucrose) present 3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time 1. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. Glucose is measured as an indicator of sucrase activity, as glucose is a producut produce by sucrase activity. 2. Explain why denatured sucrase was used as a control. Denatured sucrase was used as a control to determine if a true effect was made on the active sucrase by pH, temperature, and sucrose, or if affect would be seen in both the denatured and active sucrase.
3. Alkaline DNS denatures sucrase. Explain why it is important to denature the enzyme. It is important to determine if pH, temperature, and sucrose do have a true effect on active sucrase.
Results Table 1: Effect of pH on Sucrase Activity
1 2 3 average ph 3 0.171 0.159 0.166 0.165