ELGA
Group VI
Group Members:
Esguerra, Jezreel Legaspi, Divine Rose
Gutierrez, Joandra Pintor, Karlo
Date Submitted: March 13, 2014
Professor: Ms. Jenette Malaban
Activity 6: Fecal Analysis for the Detection of Parasites
I. Objectives:
1. T o perform the basic techniques in fecal and blood analyses.
2. To identify parasitic forms that are recognizable in the fecal samples and blood smears by microscopy.
3. To determine the advantages and limitations in each procedure.
II. Methodology Before the activity of checking or detecting the presence of parasites to the stools, it was started with getting a stool samples from the grade - IV students in Brgy. Patabog, Mulanay, Quezon.
Figure 1. Materials Needed in Stool Collection The figure on the left side is the materials usually needed in collecting the stool samples. Usually, container with formalin inside, flattened stick with line signaling for the enough amount of stool to be collected, and zip lock plastic were used in stool collection. The figure below summarizes the steps used in collecting the stool sample:
Figure 2. Steps in Stool Collection
Then different techniques in fecal analysis were used:
A. Wet Mount Preparation – Direct Fecal Smear:
Before preparing a wet mount slide, the microscope was calibrated. The objectives and oculars used for the calibration procedure were used for all measurements on the microscope. The calibration factors should always be posted on the side of the microscope. Using a wet mount identification technique, protozoan trophozoites, cysts, oocysts, and helminthes eggs and larvae may be seen and identified.
In preparing a wet mount, a clean microscope slide and the stool specimen were obtained. Then the following steps were done:
1. 1-2 drops of saline were placed at the center of the slide.
2. With and applicator stick, the various portions of the specimen was poked.
3. A smooth, uniform emulsion was made in the drop of saline by rotary
Group Members:
Esguerra, Jezreel Legaspi, Divine Rose
Gutierrez, Joandra Pintor, Karlo
Date Submitted: March 13, 2014
Professor: Ms. Jenette Malaban
Activity 6: Fecal Analysis for the Detection of Parasites
I. Objectives:
1. T o perform the basic techniques in fecal and blood analyses.
2. To identify parasitic forms that are recognizable in the fecal samples and blood smears by microscopy.
3. To determine the advantages and limitations in each procedure.
II. Methodology Before the activity of checking or detecting the presence of parasites to the stools, it was started with getting a stool samples from the grade - IV students in Brgy. Patabog, Mulanay, Quezon.
Figure 1. Materials Needed in Stool Collection The figure on the left side is the materials usually needed in collecting the stool samples. Usually, container with formalin inside, flattened stick with line signaling for the enough amount of stool to be collected, and zip lock plastic were used in stool collection. The figure below summarizes the steps used in collecting the stool sample:
Figure 2. Steps in Stool Collection
Then different techniques in fecal analysis were used:
A. Wet Mount Preparation – Direct Fecal Smear:
Before preparing a wet mount slide, the microscope was calibrated. The objectives and oculars used for the calibration procedure were used for all measurements on the microscope. The calibration factors should always be posted on the side of the microscope. Using a wet mount identification technique, protozoan trophozoites, cysts, oocysts, and helminthes eggs and larvae may be seen and identified.
In preparing a wet mount, a clean microscope slide and the stool specimen were obtained. Then the following steps were done:
1. 1-2 drops of saline were placed at the center of the slide.
2. With and applicator stick, the various portions of the specimen was poked.
3. A smooth, uniform emulsion was made in the drop of saline by rotary