Effects of Temperature on Catalase

Better Essays
QUEENSLAND ACADEMY
FOR HEALTH SCIENCES
INTERNATIONAL BACCALAUREATE
Term 1 2013
SUBJECT: BIOLOGY YEAR 11 HL & SL

Name of Student:

Name of Teacher:

FORMATIVE ASSESSMENT ITEM: Chemistry of Life Practical Investigation Term 1

Task: Investigation of a Factor Affecting Enzyme ActionAspects Assessed: D DCP CE MS Introduction:Thousands of enzymes are found in living cells. In addition to making life possible, many enzymes have numerous applications that affect our daily lives in other ways such as food processing, clinical diagnoses, sewage treatment, and the textile industry. In this activity you will investigate how a particular factor influences the action of an enzyme. Conditions: Students are NOT required to design an experiment. Experimental design has been provided for you to use instead. You are however required to submit a Risk Assessment for the investigation. Students are to work independently. However, you will need to collect raw data from 4 other students to make the 6 trials. The final version is to be submitted to TURNITIN and then class teacher. A journal will need to be included as part of the final submission. Time Allowed: 4 weeksDue Dates Task Out first lesson week1, term 1, 2013Risk Ass Due: last Lesson week 2, Term 1, 2013Final: First lesson week 6, Term 1, 2013Procedure: Your task is to investigate the effect of temperature on the activity of enzyme catalase found in beef liver. Use the experimental design and the BioLab Guide provided to assist with your report write up. Safety Considerations: You must have the risk assessment checked before continuing. NOTE: For this task you are required to submit a journal which documents your progress. You are also required to include photographs which display the techniques you used in the experimental



References: Damon, Alan, Randy McGonegal, Patricia Tosto, and William Ward. Pearson Baccalaureate: Standard Level Biology for the IB Diploma (Pearson International Baccalaureate Diploma: International Editions). n/a: Imprint Unknown, 2008. Print. “Effect of Catalase on Hydrogen Peroxide.” http://www.sciencegeek.net/Biology/biopdfs/Lab_Catalase.pdf. (25 Jan. 2013). (Add your own references)

You May Also Find These Documents Helpful

  • Powerful Essays

    Report The effect of temperature on the reaction between Catalase and H2O2 Sarah AlShemesi In this experiment we’ll be exploring the effects of temperature on the reaction between Catalase and H2O2.We’ll be using five different temperatures to test this. The five different temperatures will be 10, 30, 50, 70 and 90 oC. We will use the liver as a source of Catalase. A 1 gram piece of liver will be inserted into a test tube with 2 cm3 of water, then 2 cm3 of H2O2 will be added. The Catalase and H2O2…

    • 1721 Words
    • 7 Pages
    Powerful Essays
  • Better Essays

    The Effect of Temperature on the Enzyme Catalase Stephen Francis Biology 183 Abstract This experiment was performed to determine the resultant effect of temperature change on the reaction between the enzyme catalase and hydrogen peroxide. This experiment was performed by measuring and comparing the amount of oxygen bubbles produced and the absorbance of the catalase and hydrogen peroxide solution over time at room temperature, 2°C, 50°C, and 60°C. The overall result of this experiment proved…

    • 3527 Words
    • 15 Pages
    Better Essays
  • Satisfactory Essays

    test for the effects of temperature on catalase activity. Introduction: Enzymes are proteins that act as catalysts and help complex reactions to occur everywhere in life. Enzymes affect a chemical reaction by acting as a catalyst, which is to say, it speeds up the reaction. However, extreme ph and temperature hinders enzyme function. Optimal activity of an enzyme is the optimal environment that an enzyme requires to function, which means having the optimum ph and temperature to catalyze a…

    • 575 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    find out the effects of the temperature of enzyme catalase. My hypothesis was correct that the highest temperature of enzyme would have the least reaction of the bubble, according to the data. Referring to the data, the highest temperature of hydrogen peroxide (95.8) caused the least reaction of bubbles, which indicates enzyme and the substrate has to fit together in the active site, in order for enzyme to keep its conformation. However, as the temperature increases, the highest temperature of the enzyme…

    • 107 Words
    • 1 Page
    Good Essays
  • Good Essays

    Effect of Catalase at Different Temperatures Abstract The role of this experiment is to determine the effect of temperature on enzymatic activity. The results of the experiment were the colder the temperature the slower the reaction rate and the hotter the temperature the faster the reaction rate. Introduction Enzymes are chemical substances found in living cells and they act as catalysts of the various chemical reactions that occur in them.(Preszler, 2012) They bind to substrates that have…

    • 506 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    THE EFFECT OF TEMPERATURE ON CATALASE RESEARCH QUESTION: What is the effect of various temperatures, 0°C, room temperature, 37°C, 50°C, 60°C, on the number of oxygen gas bubbles liberated, in a decomposition reaction between the enzyme Catalase, obtained from crushed mung beans, and 2% of the substrate Hydrogen Peroxide? INTRODUCTION: Enzymes are biological catalysts that increase the rate of chemical reactions without they themselves being involved in the reaction itself. Enzymes are proteins…

    • 849 Words
    • 4 Pages
    Satisfactory Essays
  • Better Essays

    investigate the effect of temperature on the enzyme catalase. The original research question was exploring the effect temperature would have on a yeast catalase reacting with hydrogen peroxide (H2O2). To address the latter question a series of experiments were conducted. The various temperatures experimented with were as follows: 22 degrees Celsius (room temperature), 0 degrees Celsius (freezing), 100 degrees Celsius (boiling), and 37 degrees Celsius. Along with variations in temperature, the experiment…

    • 1889 Words
    • 6 Pages
    Better Essays
  • Satisfactory Essays

    DNA Structure Monday, 15 April 2013 9:01 AM - DNA = Deoxyribonucleic acid DNA is a double-helix: it has two strands that twist around each other Each strand is made of single units call nucleotides It has a sugar-phosphate backbone Bases join the two strands by hydrogen bonds ○ These bases are cytosine, guanine, adenine and thymine. - Complementary base pairing is a key idea in genetics: C pairs with G, and T pairs with A. - Each strand of DNA can be millions of base pairs in length and is…

    • 508 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Catalase Temperature Lab

    • 740 Words
    • 3 Pages

    I was testing different temperatures and how it affects the time catalase takes for it to break down peroxide. The problem that i was investigating is how does temperature affect the time it takes for catalase to break down peroxide. My independent variable for this lab was the temperature of the solution the enzyme is in. My dependent variable in this experiment was rate of reaction or the amount of time it takes to sink and rise. My hypothesis is that if the temperature is higher than 37℃, then…

    • 740 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    show that as the temperature increases, the rate of reaction between catalase and hydrogen peroxide also increased until the optimum temperature was reached. Once the temperature exceeded this optimum temperature the rate of enzyme activity had decreased. The optimum temperature for catalase in this experiment was 40°C which gave the maximum rate of reaction. From 0°C to 40°C the height of the froth increased resulting in a peak of the height of froth at 64mm. As the temperature was increased molecules…

    • 239 Words
    • 1 Page
    Satisfactory Essays