Effects of hyperventilation on CO2 and pH levels
Physiology Lab 142
Aprill 25, 2014
The aim of this experiment was to analyze how pH and carbon dioxide (CO2) levels in the blood change during hyperventilation and how the renal system compensates for this condition. Hyperventilation decreases CO2, increases pH, and lowers H+ levels which then results to respiratory alkalosis. In the experiment, we used the PhysioEx 8.0 software to simulate hyperventilation and recorded the change of the breathing pattern which gave us the levels of pH and CO2. The first run of hyperventilation results signified alkalosis. On the second hyperventilation run, the breathing pattern went from being in the alkalosis state then gradually went back to normal. A correlation graph with a R2 value was also used which showed that the relationship of PCO2 and pH is almost perfectly linear as well as the predictions. It is important to know how CO2 and pH levels affect body fluids and also the essential factors that would initiate respiratory alkalosis in order to know if there are more serious causes of hyperventilation that exist. Introduction
Respiratory alkalosis happens when the respiratory system acquires too less carbon dioxide in the blood (Health Guide). Respiratory alkalosis is the result of being on an elevated area and could be further brought on by anxiety or fever. This state is also known as hyperventilation, where carbon dioxide is released from body fluids decreasing the amount of H+ in the blood which will cause the blood’s pH level to rise (Sarikas & Cummins 422).
The PhysioEx 8.0 Software was used in this experiment to simulate hyperventilation. There were three parts in this experiment: Normal Breathing, Hyperventilation Run-1, and Hyperventilation Run-2. We started with Normal Breathing in order to establish a baseline data. First, we clicked the Start button and observed the trace lines. We then recorded the readings for pH at 20 seconds, 40 seconds, and 60 seconds. When the trace stopped, we clicked the Record Data button to record the results then clicked Print Graph in the Tools Menu to view the graph. Then, we clicked Clear Tracings to clear the oscilloscope screen. Part 2 was on Hyperventilation Run-1. We clicked Start and allowed the normal breathing trace to run for 10 seconds; then at the 10 seconds mark we clicked Hyperventilation. We carefully observed and recorded the readings for pH during 20 seconds, 40 seconds, and 60 seconds. As soon as the trace stopped running, we clicked Record Data. We also selected Print Graph from the Tools menu to view the graph and cleared tracings to clear the oscilloscope screen for the last part. The last part was on Hyperventilation Run-2. We clicked Start again and allowed the normal breathing trace to run for 10 seconds then clicked Hyperventilation after 10 seconds then clicked the Normal Breathing again when it reached 40 seconds. When the tracings ended, we observed and collected data about the changes in the pH meter and the PCO2 displays.
Table 1. Changes of pH and PCO2 as hyperventilation progressed
Figure 1. The graph shows the relationship between changes of pH and PCO2. It shows that over the time as the amount of PCO2 decreases, the pH level of the body fluids increases, making it alkaline. Table 2. Changes of pH and PCO2 in the figure 2
Figure 2. Changes of breathing pattern (V/time) as breathing mode changes from normal to hyperventilation and back Discussion
Carbon dioxide plays a central role in keeping the pH level of the blood. In this case, CO2 is released which caused pH to increase, H+ to decrease, and CO2 to decrease causing respiratory alkalosis. Figure 1 shows...
References: "Health Guide." Alkalosis. N.p., n.d. Web. 16 Apr. 2013.
Sarikas, Stephen N., and Kerri L.K. Cummins. Laboratory Investigations in Anatomy and Physiology. Second ed. N.p.: n.p., n.d. Print.
"Hyperventilation-Prevention." WebMD. WebMD, 03 Jan. 0000. Web. 19 Apr. 2013.
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