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Ebli Strain Lab Report

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Ebli Strain Lab Report
The overall purpose of this experiment was to induce and purify recombinant Green Fluorescent Protein in the E. coli strain BL21(DE3) (-- removed HTML --) (-- removed HTML --) by using a His6-tagging method and metal affinity chromatography. rGFP presence and activity was determined by measuring fluorescence under both UV light and by using a spectrofluorometer. The E2 elution fraction had the highest measured fluorescence of 20,899 relative fluorescent units (RFU). The specific activity and total protein amount in the fractions was measured by using the Bradford assay. Total protein amount in the E2 fraction was measured to be 47.39 g with a specific activity of roughly 441,000 RFU/mg. The purity of the fractions was then tested by using
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