Drosophila melanogaster Genetics Lab

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Christian Chou
Mrs. McCarron

12/10/14
A.P Biology
Drosophila melanogaster Genetics

Introduction:
The common fruit fly, scientifically named Drosophila melanogaster, is used by many in genetic studies. Because they can be easily cultured, have a relatively short generation time, and are prolific breeders, fruit flies are often very popular in genetic investigations. Furthermore, mutations and sex are easy to visualize among the fruit flies. Male fruit flies have a smaller abdomen than their female counterparts, and they also have sex combs, or black bristles, on their forelegs, which females lack. Genes are the molecular units of heredity in all organisms and are located on chromosomes. Linked­genes are located on the same chromosome and are inherited together. Wild fruit flies possess the dominant traits and alleles, whereas mutant fruit flies show the recessive traits and alleles. In this lab. genetics crosses will be performed on fruit flies to determine their sex and determine whether or not they have a mutation. Data will be collected from both an F and an F generation. Also to test the results of a cross between a sepia mutant
1
2 fruit fly and the normal, wild type fruit fly. Null Hypothesis: There will be no real difference between the observed results and the expected results. Materials and Procedure: Materials:
­ Culture vials with foam plugs
­ Formula 4­24 dehydrated Drosophila media
­ Dry active yeast
­ Distilled water
­ Fly Nap anesthetic Procedure:
­ See lab sheet Results/Data Collection/Analysis:

­ Petri dishes
­ Fine artist’s brush
­ Dissecting microscope
­ Fly “morgue” (DO NOT DRINK)

Table 1: Phenotypes of the parental strains of Drosophila Phenotype

Phenotype

Wild type: Red eyes, normal wings, short antennae.

Mutant A: Red eyes, vestigial wings, short antennae.

Wild type: Red eyes, normal wings, short antennae.

Mutant B: White eyes, normal wings,

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