Dna Replication Lab Report
DNA replication mechanism is an integral and the most important part of cell division. It is a complicated mechanism that requires a lot of steps and different enzymes. Many scientists were trying to understand the mechanism of DNA replication and its role in the transfer of the hereditary information to the offsprings. DNA replication begins with a single origin of replication that starts off the process by creating a replication bubble. In many eukaryotic cells, the DNA strand will have more than one origin of replication and, to speed up the process, the forming bubbles fuse together. By looking closely at the mechanism, one can identify a replication fork and many enzymes that play important roles in producing new double helices in the
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The DNA double-helix is separated into two single-stranded template strands. The strands are antiparallel to each other, running in the opposite directions. One of the strands runs in the 5′ to 3′ direction, and the other runs in the 3′ to 5′ direction. However, as we know, the DNA polymerases can only add nucleotides to the 3′ end. Despite the different directions of the parental strands, the matching strands for leading and the lagging parts are still synthesized in the 5′ to 3′ direction due to the capricious behavior of DNA polymerases (the lagging strand still requires a specific mechanism to achieve …show more content…
RNA primase plays an important role in the DNA replication. For a new DNA sequence to be synthesized and matched to a parental strand, RNA primers should first be introduced. It is usually the first step after the replication fork and the helicase have done their job. An RNA primer is made by joining RNA nucleotides; this job is performed by the RNA primase. Since both the leading strand and the lagging strand need a pair, the RNA primase is present in both mechanisms, regardless of the directionality of the strand.
If all choices were true, choice E must be the answer to the question.
A Journal Article published in 2016 discusses the effect of the long inverted repeat on DNA replication. The inverted repeats being single stranded nucleotide sequences with reverse complements were found to be causing fleeting setbacks in the mechanism of DNA replication. The reason for it is that they tend to form hairpins on DNA strands. The scientists were particularly interested in how the replication fork is affected by the products of the long inverted repeats. It was shown that the DNA replication, specifically the replication fork, is indeed transiently slowed down by the hairpins on the sequence of the leading strand; however, the effects made on the lagging strand were quite obscure. The research team proposed a solution for the extraction of the long inverted repeats from the sequence for the benefit of more precise and faster
The DNA double-helix is separated into two single-stranded template strands. The strands are antiparallel to each other, running in the opposite directions. One of the strands runs in the 5′ to 3′ direction, and the other runs in the 3′ to 5′ direction. However, as we know, the DNA polymerases can only add nucleotides to the 3′ end. Despite the different directions of the parental strands, the matching strands for leading and the lagging parts are still synthesized in the 5′ to 3′ direction due to the capricious behavior of DNA polymerases (the lagging strand still requires a specific mechanism to achieve …show more content…
RNA primase plays an important role in the DNA replication. For a new DNA sequence to be synthesized and matched to a parental strand, RNA primers should first be introduced. It is usually the first step after the replication fork and the helicase have done their job. An RNA primer is made by joining RNA nucleotides; this job is performed by the RNA primase. Since both the leading strand and the lagging strand need a pair, the RNA primase is present in both mechanisms, regardless of the directionality of the strand.
If all choices were true, choice E must be the answer to the question.
A Journal Article published in 2016 discusses the effect of the long inverted repeat on DNA replication. The inverted repeats being single stranded nucleotide sequences with reverse complements were found to be causing fleeting setbacks in the mechanism of DNA replication. The reason for it is that they tend to form hairpins on DNA strands. The scientists were particularly interested in how the replication fork is affected by the products of the long inverted repeats. It was shown that the DNA replication, specifically the replication fork, is indeed transiently slowed down by the hairpins on the sequence of the leading strand; however, the effects made on the lagging strand were quite obscure. The research team proposed a solution for the extraction of the long inverted repeats from the sequence for the benefit of more precise and faster