Dna Extraction Lab Write Up
DNA Extraction Lab
Purpose: To compare the amount of DNA extracted from two different species, despite using the same method.
Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure.
Materials:
-Sample of Strawberries -Zip lock bag
-DNA extraction buffer -Cold ethanol
-Glass rod -Double ply cheese cloth
-Two test tubes -Test tube rack
-Funnel -Sample of meat
-Two small beakers -Graduated Cylinder
Procedure: Refer to page, 224 1. Repeat procedures for a small sample of meat and a sample of fruit. 2. Place fruit sample in a zip lock bag. Remove …show more content…
The glass is positively charge and the oxygen in the DNA is negatively charged. When the two came in contact with each other, the rule of opposites attracted took place. That is why the DNA “stuck” to the glass rod.
d) The DNA we extracted was very gooey, and a sort of thick liquid. The DNA did appear flexible and that is because it is so small. The rigid double helix structure does apply but is too small to see. When we look at it from the naked eye it appears to be flexible. This is because of the hydrogen bonds in the nucleotides which allows the DNA to bend.
e) The DNA was pure except for some human errors. Such that the glass rod was clean but not totally sanitized which could lead to contamination, same as the beakers and the cheese cloth. As soon as you touch something, the dirty and particles transfer to that object. It would be very hard to keep the DNA pure especially when weighing it and dust and other airborne particles get on it. Other than that, the DNA was actually quite pure.
f) The liver gave us more DNA than the strawberry did. This is because an animal cell has more chromosomes than a plant cell does. This allows us to gather more DNA more easily than it did for the
Purpose: To compare the amount of DNA extracted from two different species, despite using the same method.
Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure.
Materials:
-Sample of Strawberries -Zip lock bag
-DNA extraction buffer -Cold ethanol
-Glass rod -Double ply cheese cloth
-Two test tubes -Test tube rack
-Funnel -Sample of meat
-Two small beakers -Graduated Cylinder
Procedure: Refer to page, 224 1. Repeat procedures for a small sample of meat and a sample of fruit. 2. Place fruit sample in a zip lock bag. Remove …show more content…
The glass is positively charge and the oxygen in the DNA is negatively charged. When the two came in contact with each other, the rule of opposites attracted took place. That is why the DNA “stuck” to the glass rod.
d) The DNA we extracted was very gooey, and a sort of thick liquid. The DNA did appear flexible and that is because it is so small. The rigid double helix structure does apply but is too small to see. When we look at it from the naked eye it appears to be flexible. This is because of the hydrogen bonds in the nucleotides which allows the DNA to bend.
e) The DNA was pure except for some human errors. Such that the glass rod was clean but not totally sanitized which could lead to contamination, same as the beakers and the cheese cloth. As soon as you touch something, the dirty and particles transfer to that object. It would be very hard to keep the DNA pure especially when weighing it and dust and other airborne particles get on it. Other than that, the DNA was actually quite pure.
f) The liver gave us more DNA than the strawberry did. This is because an animal cell has more chromosomes than a plant cell does. This allows us to gather more DNA more easily than it did for the