Dna Extraction Lab Report
DNA extraction lab
1. A number of steps are required to isolate DNA from cellular content. Describe what happens at each step, and why it acts to separate the parts of the cell.
The steps include a) breaking cell open to release the DNA; b) separating the DNA from cellar materials and proteins; c) using alcohol to precipitate the DNA; d) cleaning the DNA; e) confirming the presence of the DNA.
a) Breaking cell open to release the DNA: the cells are separated from each other by physical means such as grinding and transported into a salty solution. The salty solution protects the DNA from unexpected harm. The detergent is then added to break down the lipids in the cell membrane and nuclie. DNA is then released.
b) separating the DNA from cellular materials and proteins: a protein enzyme is used to take down DNA associated proteins. Filters are used to remove the cellular materials.
c) using the alcohol: ethanol is often added to the sample; adding the alcohol helps, a dna can be made visible; DNA is insoluble in alcohol and in the presence of the salt;
d) cleaning the DNA: DNA is further cleaned and put in a little bit alkaline buffer to be used;
e) confirming the presence of the DNA: the concentration and quality of the DNA have to be determined; this can be …show more content…
Yet the extraction process is different. The amount of DNA depends on the size and type of the cell. Certain cells contain more DNA than others. Plant cells are different from the animal cells because of their cell wall. Some plants have more than one copy of each chromosome per cell. Animal cells don't have a wall. Yet all of them have to be treated with chemicals to disrupt the cell membrane. The main difference between plant and animal DNA is in the sequence of bases in the helix. There are different compounds in the plant and animal DNA. The plant DNA is often larger than the animal one. These differences affect the extraction as they require different levels of
1. A number of steps are required to isolate DNA from cellular content. Describe what happens at each step, and why it acts to separate the parts of the cell.
The steps include a) breaking cell open to release the DNA; b) separating the DNA from cellar materials and proteins; c) using alcohol to precipitate the DNA; d) cleaning the DNA; e) confirming the presence of the DNA.
a) Breaking cell open to release the DNA: the cells are separated from each other by physical means such as grinding and transported into a salty solution. The salty solution protects the DNA from unexpected harm. The detergent is then added to break down the lipids in the cell membrane and nuclie. DNA is then released.
b) separating the DNA from cellular materials and proteins: a protein enzyme is used to take down DNA associated proteins. Filters are used to remove the cellular materials.
c) using the alcohol: ethanol is often added to the sample; adding the alcohol helps, a dna can be made visible; DNA is insoluble in alcohol and in the presence of the salt;
d) cleaning the DNA: DNA is further cleaned and put in a little bit alkaline buffer to be used;
e) confirming the presence of the DNA: the concentration and quality of the DNA have to be determined; this can be …show more content…
Yet the extraction process is different. The amount of DNA depends on the size and type of the cell. Certain cells contain more DNA than others. Plant cells are different from the animal cells because of their cell wall. Some plants have more than one copy of each chromosome per cell. Animal cells don't have a wall. Yet all of them have to be treated with chemicals to disrupt the cell membrane. The main difference between plant and animal DNA is in the sequence of bases in the helix. There are different compounds in the plant and animal DNA. The plant DNA is often larger than the animal one. These differences affect the extraction as they require different levels of