DNA Extraction

Good Essays
Republic of the Philippines
Mindanao State University
General Santos City

Bio 108: Cell Biology
Laboratory Report

August 28, 2013

DNA Extraction on Onion (Allium cepa) and Human Cheek Cell

Arriza, Rolland Merch
Buscato, Carl G.
Butil, Conrad G.
Leonida, Renee Theresa

ABSTRACT
This activity observes the DNA present in onion (Allium cepa) and human cheek cells by extracting it with the addition of lysis buffer and chilled ethanol. The lysis buffer is prepared from squeezed onion mixed with salt and Pantene Pro-V Shampoo. The buffer degrades the protein enveloping the DNA found in onion and cheek cells Cheek cells are obtained from gargled gatorade. Extracts are then filtered and subjected to addition of chilled ethanol. DNA is insoluble in alcohol making it to precipitate, DNA is then extracted.

Keywords: DNA extraction, onion, human cheek cell, lysis

INTRODUCTION

DNA Extraction is the removal of deoxyribonucleic acid (DNA) from the cells or viruses in which it normally resides. Extraction of DNA is often an early step in many diagnostic processes used to detect bacteria and viruses in the environment as well as diagnosing disease and genetic disorders. The process of isolating DNA from a cell is the first step for many laboratory procedures in biotechnology. The scientist must be able to separate the DNA from the unwanted substances of the cell gently enough so that the DNA is not broken up or shredded. The procedure you will be doing is a modification of the "Marmur" preparation which is used worldwide in biotechnology laboratories.

A "Filtrate" is made of onions treated with salt, distilled water and detergent(SDS). An onion is used because it has a low starch content which allows the DNA to be seen more clearly. The salt shields the negative phosphate end of DNA which allows these ends to come closer so they can precipitate out of a cold alcohol solution. The detergent causes the cell membrane to breakdown by



References: http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/DNA-RNA-Purification-Analysis/dna-extraction/genomic-dna-extraction/plant-dna-extraction.html http://www.accessexcellence.org/AE/AEC/AEF/1994/dollard_onionDNA.php

You May Also Find These Documents Helpful

  • Satisfactory Essays

    RPT Extraction of DNA

    • 287 Words
    • 1 Page

    C. What was the purpose of adding the detergent to the experiment? If the detergent was not used, do you think the experiment results would have changed? Explain your answer.…

    • 287 Words
    • 1 Page
    Satisfactory Essays
  • Better Essays

    DNA extraction lab

    • 1830 Words
    • 6 Pages

    "Ethanol Precipitation of DNA and RNA: How it works." Bitesize Bio. N.p., n.d. Web. 20 Feb.…

    • 1830 Words
    • 6 Pages
    Better Essays
  • Satisfactory Essays

    Strawberry dna lab

    • 731 Words
    • 3 Pages

    Background information: The long thick fibers of DNA store the information for the function of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found can be extracted using common, everyday material. We will use an extraction buffer containing salt, to break up protein chains that bind around the nucleic acids, and dish soap to dissolve the lipid (fat) part of the strawberry cell wall and nuclear membrane. This extraction buffer will help provide us access to the DNA inside the cells.…

    • 731 Words
    • 3 Pages
    Satisfactory Essays
  • Better Essays

    Nt1310 Unit 1 Exercise 1

    • 1475 Words
    • 6 Pages

    Cell and molecular biology is a science based on the various systems of a cell resulting to its regulation, maintenance and function. Many of these systems involve genetic information hence the study of the DNA is an essential part of this field. To able to analyze DNA, it must first be isolated and purified from its natural environment filled with biological molecules and compounds that cause physical or chemical interference in an experimental set-up. Several protocols have been established to efficiently extract DNA, one of which, the CTAB method, was performed and studied in this exercise. (De la Parte and Dita, 2014)…

    • 1475 Words
    • 6 Pages
    Better Essays
  • Good Essays

    In this experiment we used dried split peas to extract the DNA and observed the protein isolate.…

    • 520 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Ap Bio Essay Example Biotech

    • 2809 Words
    • 12 Pages

    PART B. Describe the results you would expect from electrophoretic separation of fragments from the following treatments of the DNA segment above.…

    • 2809 Words
    • 12 Pages
    Better Essays
  • Good Essays

    Ambrosia Beetle Hypothesis

    • 1318 Words
    • 6 Pages

    DNA extraction of the fungi. The sequence will be compared to the sequences obtained by…

    • 1318 Words
    • 6 Pages
    Good Essays
  • Powerful Essays

    The aim of the experiment is to isolate purified genomic DNA from cheek cells, prepare the sample for PCR reaction, place prepared sample in agarose gel through a procedure called…

    • 2156 Words
    • 9 Pages
    Powerful Essays
  • Good Essays

    Biology

    • 696 Words
    • 3 Pages

    sample of DNA obtained / leucocytes / from mouthwash / hair / other named source…

    • 696 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Dna Dna the Money

    • 301 Words
    • 2 Pages

    Long stands of double helical DNA can fit into the nucleus of a single cell because DNA is specially packaged through a series of compaction events to fit easily within cell nuclei. Even though the length of DNA per cell is about 100,000 times as long as the cell itself, it only takes up only about 10 percent of the cell’s volume. The DNA molecule, in order to condense, wraps itself around groups of histone proteins, and then the chromatin folds back on it, nucelosomes pack together to create a compact, protein-coated fiber, and the fiber coils to shorten further into an extended chromosome. Finally, the coiled fiber organizes into loops coming from a central axis, creating a condensed, X-shaped chromosome.…

    • 301 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    Strawberry Extraction Lab

    • 745 Words
    • 3 Pages

    DNA is the genetic information in organisms that codes for proteins, and controls the cells function. The DNA is a double helix molecule that is formed by various nucleotides which include Adenine, Thymine, Guanine, and Cytosine with sugar phosphate. Different organisms can have larger or smaller genes. DNA is also what makes up chromosomes during cell replication. Strawberries have a large amount of genes. The extraction solution which includes soap and salt is used to disrupt the cell’s plasma membrane and nuclear envelope, to make the DNA clump together. The salt breaks up the protein chains that bind the nucleic acids. DNA does not dissolve in ethanol, and the colder the ethanol is, the less DNA that will be broken down allowing us to view the DNA in the test tube.…

    • 745 Words
    • 3 Pages
    Satisfactory Essays
  • Satisfactory Essays

    If we put onion root tips in IAA solution then the rate of Mitosis will increase.…

    • 528 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    If you add certain chemicals to the beaten cells of a strawberry the sticky DNA can be retrieved from within the cells.…

    • 667 Words
    • 3 Pages
    Good Essays
  • Good Essays

    bio 243 lab final notes

    • 1177 Words
    • 5 Pages

    · To extract the DNA, isopropanol was added which decreased the polarity of the solvent. The DNA was then extracted by allowing the DNA to wind around a glass rod.…

    • 1177 Words
    • 5 Pages
    Good Essays
  • Better Essays

    In the experiment we were inspecting the amount of genetic materials in fruits with different numbers of chromosomes. In the experiment, we mashed up a strawberry/kiwi fruit (See appendix 1 figure 2) added extraction butter and ethanol to extract the DNA. A chromosome is a long thread-like cluster of genes in the nucleus. DNA (deoxyribonucleic acid), a self-replicating material which is present in nearly all living organisms as the main constituent of chromosomes. It is the carrier of genetic information. Genes, a unit of heredity which is transferred from a parent to offspring and is held to determine some characteristic of the offspring. The location of DNA is in a cell’s nucleus and the structure is in a shape like a coiled ladder, the sides of the ‘ladder’ are made up of alternation and the ‘ladder rings’ are pairs of nitrogen bases. The purpose of DNA is to control your development which dictates your eye colour, bone structure, blood type basically everything about you, but can be passed down to the next descendant. DNA extraction is a routine procedure to collect DNA for subsequent molecular or forensic analysis.…

    • 802 Words
    • 4 Pages
    Better Essays

Related Topics