The purpose of this lab was to understand how different solutions played a role in the digestion protein. By looking at different variables, such as temperature, and pH we’re capable of understanding just how certain substances functioned and when they didn’t. The data for all labs are clear and concise and give a clear understanding of what solutions work best. All three labs were placed in a warm water bath set at 37’C to stimulate the reaction as if it were taking place within the human body. This gives us a more accurate reading on how they would react at that set temperature. We concluded why certain tubes changed to the color they did and further explained it. This lab focuses primarily on two crucial components of digestion; 1) The actual solutions involved in digestion and how they react alone and paired and 2) Acidity of the solution since our stomachs are unbelievably acidic.
In these labs, my partner and I had to understand, and make note of, the types of reactions that occurred in each test tube. These reactions would be brought on by enzymes that would interact with other substances. [Part A] In this lab, we hypothesized that the tubes that contained a pancreatic solution would dissolve the starch, while the other test tubes wouldn’t. This is because the pancreatic solution contains enzymes which complete the digestion of carbohydrates, proteins, and lipids (Digestion BioKit). Although test tube three contained this solution, we predicted that there wouldn’t be a reaction due to the fact that it was being boiled. The number, type, and arrangement of the amino acids determine the shape of the protein which directly controls the protein's function. The shape and function of a protein can be altered by a process known as denaturing. Too high a temperature or change of pH can cause the denaturing to take place. If this happens, then the protein can be rendered useless, unable to perform its normal job. In this case, boiling the enzyme caused the protein to become denatured and as a result, not function properly (Litehall, Sue). [Part B] In this lab, we added litmus milk, which contains azolitmin, to both of the test tubes being used. Azolitmin is a pH indicator that turns purple between pH of 4.6 and 8.2. It turns pink when pH reaches 4.5 and blue at a pH of 8.3. The purpose of this lab was to understand acid production and understand why there was a certain color change in one tube as opposed to the other tube. We hypothesized that test tube one would result in a purple color change because it has pancreatin powder, which is a component in digestion (acidic). We also believed that test tube two would be more basic since its distilled water and as a result, turn a pinkish color. [Gastric Digestion] In this lab, we added albumin powder into each test tube; Albumin is a substance that is rich in protein (Digestion BioKit). This powder was selected because the liquids that were chosen for each individual test tube play a role in the digestion of protein. For this reason, the tubes were also placed in water with the temperature being 37’C. This replicated the temperature of the human body, further limiting variables in the experiment. Our hypothesis was that test tube one wouldn’t have any reaction because when it acts alone its inactive. This led us to believe that the color of the liquid would turn purple. For test tube two, we believed that it would also not have a reaction because we felt that HCl needed to act off of the pepsin solution. One without the other wouldn’t lead to the digestion of protein. Our final hypothesis, for test tube three, was that there would be digestion of proteins since both the HCl and pepsin solutions are within the gastric juice. When both components are together, we felt that digestion would be capable of beginning. As a result, we believed that liquid would turn pink. Materials and Methods
Cited: - “Digestion BioKit” Carolina Biological Supply Company. 2001
- Denaturing and Protein Function: Student Activity. Frontiers Summer Research Program 1996
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