Lab Partner(s): Alexis Clouting
Date: 2/15/15
Abstract: In the content of the Module 2 we learned about Diffusion across cell membranes. We touched on the different types of cells and their functions. How things are transported in and out of cells. Learning about isotonic, hypertonic and even hypotonic solution. This is not my first time touching on this subject in my nursing career and I learned a way to remember what happens in the different solutions. This project was to see which solution was able to pass through the semi permeable membrane. I came to the conclusion that as we learned in this module some molecules are too large to pass through membranes and some are some are small enough to pass …show more content…
Measure and pour 50 mL of water into a 100 mL beaker. Cut a piece of dialysis tubing 15.0 cm long. Submerge the dialysis tubing in the water for at least 10 minutes. I measured and poured 82 mL water into a second 100 mL beaker. While the dialysis bag was soaking, made the glucose/sucrose mixture. Using a graduated pipette to add five mL of glucose solution to a third beaker and labeled it “Dialysis bag solution”. Used a different graduated pipette to add five mL of starch solution to the same beaker. Mixing and pipetting the solution up and down the pipette six times. Used the same pipette that I used to mix the dialysis bag solution, removing two mL of that solution and placed it in a clean beaker. That sample served as my positive control for glucose and starch. Dipped one of the glucose test strips into the two mL of glucose/starch solution in the third beaker. After one minute had passed, recorded the final color of the glucose test strip. Used a pipette to transfer approximately 0.5 mL of IKI to into the two mL of glucose/starch solution in the third beaker. After one minute had passed, recorded the final color of the glucose/starch solution. I used a clean pipette, and removed two mL of water from the 82 mL of water and placed it in a clean beaker. I dipped one of the glucose test strips into the two mL of water in the beaker. After one minute had passed, recording the final color of the glucose test strip. Used a pipette to transfer approximately 0.5 mL of IKI to into the two mL of water in the beaker. After one minute had passed, recorded the final color of the water in the beaker After at least 10 minutes had passed, remove the dialysis tube and close one end by folding over 3.0 cm of one