Preview

Differential Staining Lab Report

Good Essays
Open Document
Open Document
639 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Differential Staining Lab Report
Michele Hindmarsh mhindma@my.wgu.edu Student ID# 000383032
MLT1 – Experiment 5; Task 6
Differential Staining
Heidi Atkinson, MS

Lab Experiment #5-Differential Staining

Through the process of differential staining, there are distinct differences between the cell walls of gram-positive and gram-negative bacteria. In the case of gram-positive bacteria, the cell wall is comprised of 60-90% peptidoglycan and is very thick. There are numerous layers of teichoic acid bound with peptidoglycan thereby creating very thick cell membranes which causes the cell wall to take up large quantities of basic dye and appears purple. (Hands-on-Labs. (2012)). Conversely, gram-negative bacteria cell walls are much thinner with an outer cell membrane composed of phospholipids and only
…show more content…
This bacteria exists in many popular foods including yogurt, cheese, and the fermentation of beer and wine. Alternatively, the bacteria can have negative impact on the human body in the form of infections, commonly involving the urinary tract. Another gram-positive bacteria, Staphylococcus, exists in the nose and on the skin of humans. Found in these locals, it is generally benign, until there is a disruption or injury in the skin that introduces it systemically. (Stoppler, Melissa Conrad MD). Staphylococcus can be insidious and potentially life-threatening if left untreated, causing sepsis if left to run rampant. A yeast delineated as S. cerevisiae appeared purple in this experiment. This would lead one to believe that it is gram-positive as well. However, as a fungus, the rules do not apply, as its cellular structure is different and it takes up whatever color is first introduced. The only traceable source of infection linked to S. cerevisiae is via the use of S. saccromyces as a probiotic during the course of intensive antibiotic treatment and the subsequent proliferation of S.

You May Also Find These Documents Helpful

  • Good Essays

    According the Wallstreet journal, the purchase of carpet is one of the most expensive purchases that people make in their lifetime, yet this product as remained mostly undermarketed with only 5 million dollars spent on customer advertising for 15 billon dollars in revenue for the industry.…

    • 1418 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Biology Lab Report

    • 689 Words
    • 3 Pages

    My hypothesis is if the water temperature is hot then the life saver will dissolve quicker because the hot water has a greater chemical effect on the life saver than the other temperatures. I believe this is because the hot water is creating a chemical change and is changing the solid object into a liquid.…

    • 689 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Unknown Lab Report

    • 1149 Words
    • 5 Pages

    Prepare two slides for gram staining and viewing under a microscope. {Either my gram-stain slides were bad or the microscopes I chose for viewing were not good. No bacteria were found under the microscope.}…

    • 1149 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Mycoplasma are the smallest known bacteria. They are so small that they will pass through filters that most bacteria could not pass through. Mycoplasma does not have a cell wall, which allows for it to take many different shapes. The lipids in the plasma membrane in the cell creates sterols, which protect it from rupture. The peptioglyan proteins are a target for antimicrobial activity. It disrupts the final linking of the peptidoglycan rows, which leads to it's destruction due to rupture (Tortora, 2010, pg 85). Mycoplasma are gram positive when stained.…

    • 637 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Bacteria Ecology

    • 744 Words
    • 3 Pages

    To identify the resulting colonies a procedure called gram stain is used in the identification of bacteria. Bacteria can be gram-positive, gram-negative, or gram-variable. Most bacteria however, are usually gram-positive or gram-negative. Gram-positive and gram-negative bacteria react differently to staining because of the differences in their cell walls. Bacterial cell walls contain a polymer called peptidoglycan. Gram-positive bacteria have more of this polymer than gram-negative bacteria. They are also less complex than gram-negative cell walls, missing different polysaccharides, lipids, and proteins that are found in gram-negative bacteria. Three different stain types are used in the identification process: safranin (pink/red), crystal violet (purple), and Gram iodine. Along with being either gram-positive or gram-negative, most bacterial cells can be classified according to three shapes: bacilli (rods), cocci (spheres), and spirilla (spirals, or corkscrews).…

    • 744 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Biology Lab Report

    • 688 Words
    • 3 Pages

    In the 1850s, two scientists by the name of Charles Darwin and Alfred Russell Wallace composed the theory of evolution by natural selection. (1) Darwin characterized several claims needed for natural selection to happen, including heritable variation within the population, and the presence of more individuals than the environment can support. They also discovered that certain environments favored certain traits. These circumstances resulted in the preferred traits being the most adaptable and able to reproduce, therefore passing the traits down to the next generation. To get a closer look at natural selection, we set up simulations to look at the reproductive success of an individual relatives to others, or biological fitness. The hypothesis we concluded for the simulations is that the predators on the light towel bench, which represents one of our three environments, would be more fit than the predator in the other two environments.…

    • 688 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Choose an appropriate number scale for the Y-axis and label it Number of Infections. Choose an appropriate number scale for the X-axis label it Number of Exchanges.…

    • 359 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Biology Lab Report

    • 386 Words
    • 2 Pages

    They view tiny specimen of evidence under the microscope which include skin cells, hair, soil, ink, etc...…

    • 386 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Unknown Bacteria Essay

    • 614 Words
    • 3 Pages

    Secondly both bacteria had to be gram stained, so that they could be identified as gram-positive or as gram-negative. The same gram staining method was used for both bacteria, which consisted of inoculating each bacteria into a…

    • 614 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Unknow Lab Report

    • 1319 Words
    • 6 Pages

    The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining, Capsule Staining, Endospore Staining, and Acid Fast staining. One of the most significant method of staining is the Gram Staining, as it is highly dependent (McCarthy, 25). In the specific experiment that was done, Gram Staining was used and the bacteria that was found was purple and round (cocci) shaped. Through this the bacteria was identified as Staphylococcus epidermis.…

    • 1319 Words
    • 6 Pages
    Good Essays
  • Satisfactory Essays

    The bacterial cell wall consists mostly of a chemical called peptidoglycan, which is a protein sugar molecule which is an essential need for the bacteria to survive. Plant cell walls contain cellulose, pectin and hemicellulose and have two cell walls, a primary wall that is thin and flexible, and a secondary wall that is thick and contains lignin. Both the bacterial and plant cell wall are there to surround and protect them from the environment.…

    • 374 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    One possible source of contamination may be the produce that is grown and distributed locally at a farmer’s market. A test sample of some of the produce revealed evidence of bacterial contamination. The bacteria sample was tested in a microbiology lab and showed the results that follow. The laboratory also performed a Gram stain of the isolated bacteria and ran a number of biochemical tests to aid identification. The biochemical tests were assayed using the Analytical Profile Index (API) 20E system for identification of Enterobacteriaceae and other gram-negative…

    • 891 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Pseudomonas Lab

    • 862 Words
    • 4 Pages

    Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had, was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane, periplasmic space, peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any colony pigment. It appeared as a circular, raised, undulated bacterium. The arrangement of Pseudomonas aeruginosa is rod shaped. Multiple biochemical tests can be performed to determine if gram positive or negative. See Table below:…

    • 862 Words
    • 4 Pages
    Good Essays
  • Good Essays

    It can range anywhere for 20 to 80 micrometers thick. Gram-positive cell walls also contains tightly bound polysaccharides, such as teichoic acid. Teichoic acid is a polymer of ribitol or glycerol and phosphate. Lipoteichoic acid may also be present in gram-positive cell walls as this molecules binds to lipids in the cell membrane instead of proteins. The molecules found inside of the gram-positive cell wall function in cell wall maintenance, enlargement of the cell during cell division and allowing pathogenic cells to bind to host tissues. The cell wall of gram-positive cells adhere loosely to the membrane. There is a small space between where they join which is called periplasmic space. The periplasmic space is a temporary storage site of peptidoglycan…

    • 948 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Clearing agents are used to remove the dehydrant from the tissue. They also make tissue transparent by raising refractive index of any specimen. Clearing agents need to miscible with both alcohol and paraffin wax.…

    • 826 Words
    • 4 Pages
    Better Essays