FORMS AND BODY FLUIDS USING THE HIGH PERFORMANCE LIQUID
CHROMATOGRAPHY (HPLC) TECHNIQUE
S. Asare-Nkansah and J.K. Kwakye
Department of Pharmaceutical Chemistry,
Faculty of Pharmacy and Pharmaceutical Sciences,
Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
High Performance Liquid Chromatography has been used to evolve an analytical procedure for the evaluation of the content of paracetamol in the bulk, dosage forms and in urine, a body fluid. Separation and resolution have been achieved with a combination of methanol and 2.5% acetic acid (15:85) on a reversed-phase column at ambient temperature. Elution was isocratic with UV detection at 257nm.
Internal standard calibration method was used for quantitation with caffeine as the internal standard.
Mean retention times for paracetamol and caffeine were respectively 2.61 ± 0.13 min and 11.98 ± 0.72 min . The calibration curve was linear over the range 0.1-5.0μg/ml. The method was also suitable for the assay of paracetamol-codeine combination drug as well as estimation of the amount of constituents in urine when the wavelength of UV detection was 245 nm with acetanilide as the internal standard.
Keywords: Chromatography, isocratic, internal standard, in vivo and in vitro
Paracetamol (N-(4-hydroxyphenyl) acetamide) tablets are listed among the essential drugs selected for the health care delivery system in Ghana.
Figure 1: Chemical Structure of Paracetamol
Paracetamol is very much used for antipyresis and analgesia without prescription. The drug is useful in mild to moderate pain such as headache, myalgia and postpartum pain. It is a very good alternative for mild to moderate pain in patients who cannot take aspirin because of allergy, haemophilia, history of peptic ulcer and asthma. (Katzung, 1989).
As a result of the Ghana government’s policy of generic prescribing, the